An inverse relationship of rat liver folate polyglutamate chain length to nutritional folate sufficiency

Cassady, IA, Budge, MM, Healy, MJ and Nixon, PF (1980) An inverse relationship of rat liver folate polyglutamate chain length to nutritional folate sufficiency. BBA - General Subjects, 633 2: 258-268. doi:10.1016/0304-4165(80)90411-0


Author Cassady, IA
Budge, MM
Healy, MJ
Nixon, PF
Title An inverse relationship of rat liver folate polyglutamate chain length to nutritional folate sufficiency
Journal name BBA - General Subjects   Check publisher's open access policy
ISSN 0304-4165
Publication date 1980-12-01
Sub-type Article (original research)
DOI 10.1016/0304-4165(80)90411-0
Volume 633
Issue 2
Start page 258
End page 268
Total pages 11
Language eng
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
2700 Medicine
Abstract The relative concentrations of folylpolyglutamates of differing chain length in rat liver and the uptake of exogenous [3H]folic acid (20 μCi, 20 μg) into liver folylpolyglutamates were examined in rats maintained on (a) standard and folate-supplemented standard diets and (b) semi-defined folate-sufficient and folate-deficient diets. Folylpolyglutamates extracted from liver were cleaved to p-aminobenzolpolyglutamates which were separated by ion-exchange chromatography. The relative concentrations and ultimate radiolabeling of longer-chain folylpolyglutamates (six, seven and eight glutamate residues) were greatest in the livers of folate-deficient rats, whereas the intermediate-chain folylpolyglutamates (three, four and five flutamate residues) were the greatest portion of total liver folates of folate-supplemented rats. Thus, the length of the polyglutamate chain added to liver folates is inversely related to the total concentration of liver folates. These data suggest that folylpolyglutamate biosynthesis in the liver may be controlled by the liver folate concentrations. In folate insufficiency such a control mechanism would serve to enhance the affinity of folates for folate-dependent enzymes and to conserve the liver folate concentration.
Keyword (Rat liver)
Folate metabolism
Folylpolyglutamate
Nutritional sufficiency
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
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