Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions

Scott, David J., Harding, Stephen E. and Winzor, Donald J. (2015) Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions. Analytical Biochemistry, 490 20-25. doi:10.1016/j.ab.2015.08.017

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Author Scott, David J.
Harding, Stephen E.
Winzor, Donald J.
Title Evaluation of diffusion coefficients by means of an approximate steady-state condition in sedimentation velocity distributions
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-0309
Publication date 2015-12-01
Sub-type Article (original research)
DOI 10.1016/j.ab.2015.08.017
Open Access Status File (Author Post-print)
Volume 490
Start page 20
End page 25
Total pages 6
Place of publication Philadelphia, PA United States
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
This investigation examines the feasiblity of manipulating the rotor speed in sedimentation velocity experiments to generate spontaneously an approximate steady-state condition where the extent of diffusional spreading is matched exactly by the boundary sharpening arising from negative s-c dependence. Simulated sedimentation velocity distributions based on the sedimentation characteristics for a purified mucin preparation are used to illustrate a simple procedure for determining the diffusion coefficient from such steady-state distributions in situations where the concentration dependence of the sedimentation coefficient, s = s0/(1 + Kc), has been quantified in terms of the limiting sedimentation coefficient as c → 0 (s0) and the concentration coefficient (K). Those simulations have established that spontaneous generation of the approximate steady state could well be a feature of sedimentation velocity distributions for many unstructured polymer systems because the requirement that Kcoω2s0/D be between 46 and 183 cm−2 is not unduly restrictive. Although spontaneous generation of the approximate steady state is also a theoretical prediction for structured macromolecular solutes exhibiting linear concentration dependence of the sedimentation coefficient, s = s0(1 − kc), the required value of k is far too large for any practical advantage to be taken of this approach with globular proteins.
Keyword Diffusion coefficient
Sedimentation velocity
Ultracentrifugation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 11 Sep 2015, 15:47:48 EST by Prycilla Rehm on behalf of School of Chemistry & Molecular Biosciences