JIP4 is a PLK1 binding protein that regulates p38MAPK activity in G2 phase

Pinder, Alex, Loo, Dorothy, Harrington, Brittney, Oakes, Vanessa, Hill, Michelle M. and Gabrielli, Brian (2015) JIP4 is a PLK1 binding protein that regulates p38MAPK activity in G2 phase. Cellular Signalling, 27 11: 2296-2303. doi:10.1016/j.cellsig.2015.08.009

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Author Pinder, Alex
Loo, Dorothy
Harrington, Brittney
Oakes, Vanessa
Hill, Michelle M.
Gabrielli, Brian
Title JIP4 is a PLK1 binding protein that regulates p38MAPK activity in G2 phase
Journal name Cellular Signalling   Check publisher's open access policy
ISSN 1873-3913
Publication date 2015-08-17
Sub-type Article (original research)
DOI 10.1016/j.cellsig.2015.08.009
Open Access Status File (Author Post-print)
Volume 27
Issue 11
Start page 2296
End page 2303
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2016
Language eng
Abstract Cell cycle progression from G2 phase into mitosis is regulated by a complex network of mechanisms, all of which finally control the timing of Cyclin B/CDK1 activation. PLK1 regulates a network of events that contribute to regulating G2/M phase progression. Here we have used a proteomics approach to identify proteins that specifically bind to the Polobox domain of PLK1. This identified a panel of proteins that were either associated with PLK1 in G2 phase and/or mitosis, the strongest interaction being with the MAPK scaffold protein JIP4. PLK1 binding to JIP4 was found in G2 phase and mitosis, and PLK1 binding was self-primed by PLK1 phosphorylation of JIP4. PLK1 binding is required for JIP4-dependent p38MAPK activation in G2 phase during normal cell cycle progression, but not in either G2 phase or mitotic stress response. Finally, JIP4 is a target for caspase-dependent cleavage in mitotically arrested cells. The role for the PLK1–JIP4 regulated p38MAPK activation in G2 phase is unclear, but it does not affect either progression into or through mitosis.
Keyword G2 phase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
UQ Diamantina Institute Publications
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