Isolation of genetically tractable most-wanted bacteria by metaparental mating

Cuiv, Paraic O, Smith, Wendy J, Pottenger, Sian, Burman, Sriti, Shanahan, Erin R and Morrison, Mark (2015) Isolation of genetically tractable most-wanted bacteria by metaparental mating. Scientific Reports, 5 . doi:10.1038/srep13282

Author Cuiv, Paraic O
Smith, Wendy J
Pottenger, Sian
Burman, Sriti
Shanahan, Erin R
Morrison, Mark
Title Isolation of genetically tractable most-wanted bacteria by metaparental mating
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-08-21
Year available 2015
Sub-type Article (original research)
DOI 10.1038/srep13282
Open Access Status DOI
Volume 5
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
Metagenomics has rapidly advanced our inventory and appreciation of the genetic potential inherent to the gut microbiome. However it is widely accepted that two key constraints to further genetic dissection of the gut microbiota and host-microbe interactions have been our inability to recover new isolates from the human gut, and the paucity of genetically tractable gut microbes. To address this challenge we developed a modular RP4 mobilisable recombinant vector system and an approach termed metaparental mating to support the rapid and directed isolation of genetically tractable fastidious gut bacteria. Using this approach we isolated transconjugants affiliated with Clostridium cluster IV (Faecalibacterium and Oscillibacter spp.), Clostridium cluster XI (Anaerococcus) and Clostridium XIVa (Blautia spp.) and group 2 ruminococci amongst others, and demonstrated that the recombinant vectors were stably maintained in their recipient hosts. By a similar approach we constructed fluorescently labelled bacterial transconjugants affiliated with Clostridium cluster IV (including Flavonifractor and Pseudoflavonifractor spp.), Clostridium XIVa (Blautia spp.) and Clostridium cluster XVIII (Clostridium ramosum) that expressed a flavin mononucleotide-based reporter gene (evoglow-C-Bs2). Our approach will advance the integration of bacterial genetics with metagenomics and realize new directions to support a more mechanistic dissection of host-microbe associations relevant to human health and disease.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
UQ Diamantina Institute Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 06 Sep 2015, 00:15:51 EST by System User on behalf of Scholarly Communication and Digitisation Service