Single base-resolution methylome of the silkworm reveals a sparse epigenomic map

Xiang, Hui, Zhu, Jingde, Chen, Quan, Dai, Fangyin, Li, Xin, Li, Muwang, Zhang, Hongyu, Zhang, Guojie, Li, Dong, Dong, Yang, Zhao, Li, Lin, Ying, Cheng, Daojun, Yu, Jian, Sun, Jinfeng, Zhou, Xiaoyu, Ma, Kelong, He, Yinghua, Zhao, Yangxing, Guo, Shicheng, Ye, Mingzhi, Guo, Guangwu, Li, Yingrui, Li, Ruiqiang, Zhang, Xiuqing, Ma, Lijia, Kristiansen, Karsten, Guo, Qiuhong, Jiang, Jianhao, Beck, Stephan, Xia, Qingyou, Wang, Wen and Wang, Jun (2010) Single base-resolution methylome of the silkworm reveals a sparse epigenomic map. Nature Biotechnology, 28 5: 516-520. doi:10.1038/nbt.1626

Author Xiang, Hui
Zhu, Jingde
Chen, Quan
Dai, Fangyin
Li, Xin
Li, Muwang
Zhang, Hongyu
Zhang, Guojie
Li, Dong
Dong, Yang
Zhao, Li
Lin, Ying
Cheng, Daojun
Yu, Jian
Sun, Jinfeng
Zhou, Xiaoyu
Ma, Kelong
He, Yinghua
Zhao, Yangxing
Guo, Shicheng
Ye, Mingzhi
Guo, Guangwu
Li, Yingrui
Li, Ruiqiang
Zhang, Xiuqing
Ma, Lijia
Kristiansen, Karsten
Guo, Qiuhong
Jiang, Jianhao
Beck, Stephan
Xia, Qingyou
Wang, Wen
Wang, Jun
Title Single base-resolution methylome of the silkworm reveals a sparse epigenomic map
Journal name Nature Biotechnology   Check publisher's open access policy
ISSN 1087-0156
Publication date 2010-05
Sub-type Article (original research)
DOI 10.1038/nbt.1626
Open Access Status Not yet assessed
Volume 28
Issue 5
Start page 516
End page 520
Total pages 5
Place of publication New York, NY, United States
Publisher Nature Publishing Group
Language eng
Formatted abstract
Epigenetic regulation in insects may have effects on diverse biological processes. Here we survey the methylome of a model insect, the silkworm Bombyx mori, at single-base resolution using Illumina high-throughput bisulfite sequencing (MethylC-Seq). We conservatively estimate that 0.11% of genomic cytosines are methylcytosines, all of which probably occur in CG dinucleotides. CG methylation is substantially enriched in gene bodies and is positively correlated with gene expression levels, suggesting it has a positive role in gene transcription. We find that transposable elements, promoters and ribosomal DNAs are hypomethylated, but in contrast, genomic loci matching small RNAs in gene bodies are densely methylated. This work contributes to our understanding of epigenetics in insects, and in contrast to previous studies of the highly methylated genomes of Arabidopsis1 and human2, demonstrates a strategy for sequencing the epigenomes of organisms such as insects that have low levels of methylation.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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