Structural and functional characterization of dendritic arbors and GABaergic synaptic inputs on interneurons and principal cells in the rat basolateral amygdala

Klenowski, Paul M., Fogarty, Matthew J., Belmer, Arnauld, Noakes, Peter G., Bellingham, Mark C. and Bartlett, Selena E. (2015) Structural and functional characterization of dendritic arbors and GABaergic synaptic inputs on interneurons and principal cells in the rat basolateral amygdala. Journal of Neurophysiology, 114 2: 942-957. doi:10.1152/jn.00824.2014


Author Klenowski, Paul M.
Fogarty, Matthew J.
Belmer, Arnauld
Noakes, Peter G.
Bellingham, Mark C.
Bartlett, Selena E.
Title Structural and functional characterization of dendritic arbors and GABaergic synaptic inputs on interneurons and principal cells in the rat basolateral amygdala
Journal name Journal of Neurophysiology   Check publisher's open access policy
ISSN 1522-1598
0022-3077
Publication date 2015-08-01
Sub-type Article (original research)
DOI 10.1152/jn.00824.2014
Open Access Status Not Open Access
Volume 114
Issue 2
Start page 942
End page 957
Total pages 151
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Collection year 2016
Language eng
Abstract The basolateral amygdala (BLA) is a complex brain region associated with processing emotional states, such as fear, anxiety, and stress. Some aspects of these emotional states are driven by the network activity of synaptic connections, derived from both local circuitry and projections to the BLA from other regions. Although the synaptic physiology and general morphological characteristics are known for many individual cell types within the BLA, the combination of morphological, electrophysiological, and distribution of neurochemical GABAergic synapses in a three-dimensional neuronal arbor has not been reported for single neurons from this region. The aim of this study was to assess differences in morphological characteristics of BLA principal cells and interneurons, quantify the distribution of GABAergic neurochemical synapses within the entire neuronal arbor of each cell type, and determine whether GABAergic synaptic density correlates with electrophysiological recordings of inhibitory postsynaptic currents. We show that BLA principal neurons form complex dendritic arborizations, with proximal dendrites having fewer spines but higher densities of neurochemical GABAergic synapses compared with distal dendrites. Furthermore, we found that BLA interneurons exhibited reduced dendritic arbor lengths and spine densities but had significantly higher densities of putative GABAergic synapses compared with principal cells, which was correlated with an increased frequency of spontaneous inhibitory postsynaptic currents. The quantification of GABAergic connectivity, in combination with morphological and electrophysiological measurements of the BLA cell types, is the first step toward a greater understanding of how fear and stress lead to changes in morphology, local connectivity, and/or synaptic reorganization of the BLA.
Keyword GABAergic synaptic inputs
Dendritic arbors
Interneurons and principal cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2016 Collection
School of Biomedical Sciences Publications
 
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