Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide-Based Chemosensing Ensemble

Meng, Qingtao, Jia, Hongmin, Gao, Xue, Wang, Yue, Zhang, Run, Wang, Renjie and Zhang, Zhiqiang (2015) Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide-Based Chemosensing Ensemble. Chemistry - An Asian Journal, 10 11: 2411-2418. doi:10.1002/asia.201500690


Author Meng, Qingtao
Jia, Hongmin
Gao, Xue
Wang, Yue
Zhang, Run
Wang, Renjie
Zhang, Zhiqiang
Title Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide-Based Chemosensing Ensemble
Journal name Chemistry - An Asian Journal   Check publisher's open access policy
ISSN 1861-4728
1861-471X
Publication date 2015
Sub-type Article (original research)
DOI 10.1002/asia.201500690
Open Access Status Not Open Access
Volume 10
Issue 11
Start page 2411
End page 2418
Total pages 8
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag GmbH
Collection year 2016
Language eng
Formatted abstract
We described a new ensemble-approach-based chemosensor, NCH-Cu2+, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu2+ ions over other metal ions, accompanied with a 92.2 % fluorescence quenching. The decomplexation of NCH-Cu2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH-Cu2+ towards His showed a good linearity with a detection of limit at 70 nm. Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH-Cu2+ towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH-Cu2+ was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level.
Keyword Chemosensor
Fluorescence imaging
Histidine
Lysosome
Quantification
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 27 Aug 2015, 14:19:41 EST by Prycilla Rehm on behalf of School of Chemistry & Molecular Biosciences