Analysis of the genes flanking xabB: a methyltransferase gene is involved in albicidin biosynthesis in Xanthomonas albilineans

Huang, G. Z., Zhang, L. h. and Birch, R. G. (2000) Analysis of the genes flanking xabB: a methyltransferase gene is involved in albicidin biosynthesis in Xanthomonas albilineans. Gene, 255 2: 327-333.


Author Huang, G. Z.
Zhang, L. h.
Birch, R. G.
Title Analysis of the genes flanking xabB: a methyltransferase gene is involved in albicidin biosynthesis in Xanthomonas albilineans
Journal name Gene   Check publisher's open access policy
Publication date 2000
Sub-type Article
DOI 10.1016/S0378-1119(00)00320-6
Volume number 255
Issue number 2
ISSN 0378-1119
Start page 327
End page 333
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier Science BV
Collection year 2000
Language eng
Subject C1
270800 Biotechnology
780105 Biological sciences
Abstract Transposon mutagenesis and complementation studies previously identified a gene (xabB) for a large (526 kDa) polyketide-peptide synthase required for biosynthesis of albicidin antibiotics and phytotoxins in the sugarcane leaf scald pathogen Xanthomonas albilineans. A cistron immediately downstream from xabB encodes a polypeptide of 343 aa containing three conserved motifs characteristic of a family of S-adenosyl-L-methionine (SAM)-dependent O-methyltransferases. Insertional mutagenesis and complementation indicate that the product of this cistron (designated xabC) is essential for albicidin production, and that there is no other required downstream cistron. The xab promoter region is bidirectional, and insertional mutagenesis of the first open reading frame (ORF) in the divergent gene also blocks albicidin biosynthesis. This divergent ORF (designated thp) encodes a protein of 239 aa displaying high similarity to several IS21-like transposition helper proteins. The thp cistron is not located in a recognizable transposon, and is probably a remnant from a past transposition event that may have contributed to the development of the albicidin biosynthetic gene cluster. Failure of 'in trans' complementation of rhp indicates that a downstream cistron transcribed with thp is required for albicidin biosynthesis. (C) 2000 Elsevier Science B.V. All rights reserved.
Keyword Genetics & Heredity
Antibiotic And Phytotoxin
Bidirectional Promoter
Complementation
Insertional Mutagenesis
Transposition Helper Protein
Adenosylmethionine-dependent Methyltransferases
Leaf Scald Disease
Saccharopolyspora-erythraea
Pseudomonas-syringae
Pantoea-dispersa
Sequence Motifs
Dna
Insertion
Sugarcane
Detoxification
Q-Index Code C1

Document type: Journal Article
Sub-type: Article
Collection: School of Biological Sciences Publications
 
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Created: Mon, 13 Aug 2007, 12:00:29 EST