Functional similarities between heterogeneously and homogenously expressed MscL constructs

Chi, Gamma, Rohde, Paul R., Ridone, Pietro, Hankamer, Ben, Martinac, Boris and Landsberg, Michael J. (2015) Functional similarities between heterogeneously and homogenously expressed MscL constructs. European Biophysics Journal, 44 7: 589-598. doi:10.1007/s00249-015-1062-5

Author Chi, Gamma
Rohde, Paul R.
Ridone, Pietro
Hankamer, Ben
Martinac, Boris
Landsberg, Michael J.
Title Functional similarities between heterogeneously and homogenously expressed MscL constructs
Journal name European Biophysics Journal   Check publisher's open access policy
ISSN 1432-1017
Publication date 2015-08-02
Sub-type Article (original research)
DOI 10.1007/s00249-015-1062-5
Open Access Status Not Open Access
Volume 44
Issue 7
Start page 589
End page 598
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2016
Language eng
Abstract The mechanosensitive channel of large conductance MscL is a well-characterized mechanically gated non-selective ion channel, which often serves as a prototype mechanosensitive channel for mechanotransduction studies. However, there are some discrepancies between MscL constructs used in these studies, most notably unintended heterogeneous expression from some MscL expression constructs. In this study we investigate the possible cause of this expression pattern, and compare the original non-homogenously expressing constructs with our new homogeneously expressing one to confirm that there is little functional difference between them. In addition, a new MscL construct has been developed with an improved molar extinction coefficient at 280 nm, enabling more accurate protein quantification.
Keyword Construct design
Heterogeneous expression
Mechanosensitive channel
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
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