Re-evaluation of the species of hookworms infecting dogs in Central Vietnam

Ng-Nguyen, Dinh, Hii, Sze Fui, Nguyen, Van-Anh T., Van Nguyen, Trong, Van Nguyen, Dien and Traub, Rebecca J. (2015) Re-evaluation of the species of hookworms infecting dogs in Central Vietnam. Parasites and Vectors, 8 1: 1-6. doi:10.1186/s13071-015-1015-y

Author Ng-Nguyen, Dinh
Hii, Sze Fui
Nguyen, Van-Anh T.
Van Nguyen, Trong
Van Nguyen, Dien
Traub, Rebecca J.
Title Re-evaluation of the species of hookworms infecting dogs in Central Vietnam
Journal name Parasites and Vectors   Check publisher's open access policy
ISSN 1756-3305
Publication date 2015-07-28
Sub-type Article (original research)
DOI 10.1186/s13071-015-1015-y
Open Access Status DOI
Volume 8
Issue 1
Start page 1
End page 6
Total pages 6
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2016
Language eng
Formatted abstract
Background:  Differentiation of canine hookworm species is crucial from both a veterinary and public health standpoint. In Vietnam, three hookworm species, namely Ancylostoma caninum, Ancylostoma braziliense and Uncinaria stenocephala are reported to infect dogs. In light of the emerging distribution of A. ceylanicum in Asia, this study aims to re-evaluate the status of Ancylostoma in dogs in Vietnam.

Methods:  Faecal samples collected from 200 community dogs in Dak Lak province were subjected to faecal floatation for the detection of hookworm eggs. Hookworm-positive samples were subjected to a PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) assay targeting the internal transcribed spacer (ITS) region of rDNA for hookworm species identification. A subset of hookworm-positive samples was also subject to haplotype characterisation at the cytochrome oxidase-1 (COX-1) gene. Detailed morphological criteria were utilised in addition to molecular markers, to identify adult hookworms recovered from necropsied dogs.

Results:  Of 200 canine faecal samples, 111 (55.5 %) were positive for hookworm eggs on faecal flotation. Of these, 94/111 (84.7 %) were successfully amplified and assigned species status by PCR-RFLP targeting the ITS region. In total, 54.3 % (51/94) dogs harboured single infections with A. ceylanicum, 33.0 % (31/94) with A. caninum, and 12.7 % (12/94) harboured mixed infections with both A. ceylanicum and A. caninum. Adult worms recovered from necropsied dogs matched morphological description provided for A. ceylanicum, Looss (1911) for which the mediolateral and posteriolateral rays are parallel. Characterisation of the COX-1 gene placed all Vietnamese canine isolates of A. ceylanicum within the ‘zoonotic’ haplotype.

Conclusion:  Based on this information, it is apparent that the hookworms present in dogs in Vietnam are those of A. ceylanicum and not A. braziliense. Owing to the endemic nature of this significant zoonosis in dogs, the study strongly advocates for specific identification of this hookworm in human hookworm surveys.
Keyword Ancylostoma
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Veterinary Science Publications
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