New "haploid biofilm model" unravels IRA2 as a novel regulator of Candida albicans biofilm formation

Seneviratne, Chaminda Jayampath, Zeng, Guisheng, Truong, Thuyen, Sze, Sarah, Wong, Wah, Samaranayake, Lakshman, Chan, Fong Yee, Wang, Yan-Ming, Wang, Haitao, Gao, Jiaxin and Wang, Yue (2015) New "haploid biofilm model" unravels IRA2 as a novel regulator of Candida albicans biofilm formation. Scientific Reports, 5 12433.1-12433.12. doi:10.1038/srep12433


Author Seneviratne, Chaminda Jayampath
Zeng, Guisheng
Truong, Thuyen
Sze, Sarah
Wong, Wah
Samaranayake, Lakshman
Chan, Fong Yee
Wang, Yan-Ming
Wang, Haitao
Gao, Jiaxin
Wang, Yue
Title New "haploid biofilm model" unravels IRA2 as a novel regulator of Candida albicans biofilm formation
Formatted title
New "haploid biofilm model" unravels IRA2 as a novel regulator of Candida albicans biofilm formation
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-07-23
Sub-type Article (original research)
DOI 10.1038/srep12433
Open Access Status DOI
Volume 5
Start page 12433.1
End page 12433.12
Total pages 12
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
Clinical isolates of the fungal human pathogen Candida albicans are invariably diploid and heterozygous, impeding genetic study. Recent isolation of C. albicans haploids opens opportunities to apply technologies unfeasible in diploids. However, doubts remain on whether the haploids, derived from chromosome loss, can represent the diploids. Here, we use C. albicans haploids to investigate biofilm, a key virulence attribute. We conducted the first comprehensive characterization of biofilm formation of the haploids in comparison with the diploids. We demonstrate that the haploids form biofilms with essentially the same characteristics as the diploids. Screening a haploid mutant library has uncovered novel GTPase-related genes as biofilm regulators, including IRA2 that encodes an activator of the Ras GTPase. IRA2-deletion mutants develop poorly constructed biofilm in both haploid and diploid C. albicans. Our results demonstrate that the haploids are a valid model for C. albicans biofilm research and a powerful tool for uncovering novel regulators.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Dentistry Publications
 
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