Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide

Qu, Xueqi, Proell, Maren, Neuhoff, Christiane, Zhang, Rui, Cinar, Mehmet Ulas, Hossain, Md. Munir, Tesfaye, Dawit, Grosse-Brinkhaus, Christine, Salilew-Wondim, Dessie, Tholen, Ernst, Looft, Christian, Hoelker, Michael, Schellander, Karl and Uddin, Muhammad Jasim (2015) Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide. PLoS ONE, 10 3: 1-23. doi:10.1371/journal.pone.0121574

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Author Qu, Xueqi
Proell, Maren
Neuhoff, Christiane
Zhang, Rui
Cinar, Mehmet Ulas
Hossain, Md. Munir
Tesfaye, Dawit
Grosse-Brinkhaus, Christine
Salilew-Wondim, Dessie
Tholen, Ernst
Looft, Christian
Hoelker, Michael
Schellander, Karl
Uddin, Muhammad Jasim
Title Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2015-03-20
Year available 2015
Sub-type Article (original research)
DOI 10.1371/journal.pone.0121574
Open Access Status DOI
Volume 10
Issue 3
Start page 1
End page 23
Total pages 23
Place of publication San Francisco, United States
Publisher Public Library of Science
Collection year 2016
Language eng
Formatted abstract
Histone acetylation, regulated by histone deacetylases (HDACs) is a key epigenetic mechanism controlling gene expressions. Although dendritic cells (DCs) are playing pivotal roles in host immune responses, the effect of epigenetic modulation of DCs immune responses remains unknown. Sulforaphane (SFN) as a HDAC inhibitor has anti-inflammatory properties, which is used to investigate the epigenetic regulation of LPS-induced immune gene and HDAC family gene expressions in porcine monocyte-derived dendritic cells (moDCs). SFN was found to inhibit the lipopolysaccharide LPS induced HDAC6, HDAC10 and DNA methyltransferase (DNMT3a) gene expression, whereas up-regulated the expression of DNMT1 gene. Additionally, SFN was observed to inhibit the global HDAC activity, and suppressed moDCs differentiation from immature to mature DCs through down-regulating the CD40, CD80 and CD86 expression and led further to enhanced phagocytosis of moDCs. The SFN pre-treated of moDCs directly altered the LPS-induced TLR4 and MD2 gene expression and dynamically regulated the TLR4-induced activity of transcription factor NF-κB and TBP. SFN showed a protective role in LPS induced cell apoptosis through suppressing the IRF6 and TGF-ß1 production. SFN impaired the pro-inflammatory cytokine TNF-α and IL-1ß secretion into the cell culture supernatants that were induced in moDCs by LPS stimulation, whereas SFN increased the cellular-resident TNF-α accumulation. This study demonstrates that through the epigenetic mechanism the HDAC inhibitor SFN could modulate the LPS induced innate immune responses of porcine moDCs.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Veterinary Science Publications
 
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