Highly diverse community structure in a remote central Tibetan geothermal spring does not display monotonic variation to thermal stress

Yim, Lau Chui, Hongmei, Jing, Aitchison, Jonathan C and Pointing, Stephen B. (2006) Highly diverse community structure in a remote central Tibetan geothermal spring does not display monotonic variation to thermal stress. FEMS Microbiology Ecology, 57 1: 80-91. doi:10.1111/j.1574-6941.2006.00104.x


Author Yim, Lau Chui
Hongmei, Jing
Aitchison, Jonathan C
Pointing, Stephen B.
Title Highly diverse community structure in a remote central Tibetan geothermal spring does not display monotonic variation to thermal stress
Journal name FEMS Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2006-07
Sub-type Article (original research)
DOI 10.1111/j.1574-6941.2006.00104.x
Open Access Status Not Open Access
Volume 57
Issue 1
Start page 80
End page 91
Total pages 12
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
We report an assessment of whole-community diversity for an extremely isolated geothermal location with considerable phylogenetic and phylogeographic novelty. We further demonstrate, using multiple statistical analyses of sequence data, that the response of community diversity is not monotonic to thermal stress along a gradient of 52-83°C. A combination of domain- and division-specific PCR was used to obtain a broad spectrum of community phylotypes, which were resolved by denaturing gradient gel electrophoresis. Among 58 sequences obtained from microbial mats and streamers, some 95% suggest novel archaeal and bacterial diversity at the species level or higher. Moreover, new phylogeographic and thermally defined lineages among the Cyanobacteria, Chloroflexi, Eubacterium and Thermus are identified. Shannon-Wiener diversity estimates suggest that mats at 63°C supported highest diversity, but when alternate models were applied [Average Taxonomic Distinctness (AvTD) and Variation in Taxonomic Distinctness (VarTD)] that also take into account the phylogenetic relationships between phylotypes, it is evident that greatest taxonomic diversity (AvTD) occurred in streamers at 65-70°C, whereas greatest phylogenetic distance between taxa (VarTD) occurred in streamers of 83°C. All models demonstrated that diversity is not related to thermal stress in a linear fashion.
Keyword Chloroflexus
Geothermal springs
Hot springs
Roseiflexus
Synechococcus
Thermophiles
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Geography, Planning and Environmental Management Publications
 
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Created: Wed, 29 Jul 2015, 13:17:04 EST by Helen Smith on behalf of School of Geography, Planning & Env Management