Determining phosphine resistance in rust red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae) populations from Turkey

Kocak, Erhan, Schlipalius, David, Kaur, Ramandeep, Tuck, Andrew, Ebert, Paul, Collins, Pat and Yilmaz, Abdullah (2015) Determining phosphine resistance in rust red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae) populations from Turkey. Turkiye Entomoloji Dergisi, 39 2: 129-136. doi:10.16970/ted.17464

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Author Kocak, Erhan
Schlipalius, David
Kaur, Ramandeep
Tuck, Andrew
Ebert, Paul
Collins, Pat
Yilmaz, Abdullah
Title Determining phosphine resistance in rust red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae) populations from Turkey
Formatted title
Determining phosphine resistance in rust red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae) populations from Turkey
Language of Title eng
Journal name Turkiye Entomoloji Dergisi   Check publisher's open access policy
Translated journal name Turkish Journal of Entomology
Language of Journal Name tur
ISSN 1010-6960
Publication date 2015
Sub-type Article (original research)
DOI 10.16970/ted.17464
Open Access Status Not yet assessed
Volume 39
Issue 2
Start page 129
End page 136
Total pages 297
Place of publication Bornova, Izmir, Turkey
Publisher Ege Universitesi Faculty of Agriculture
Collection year 2016
Language eng
tur
Formatted abstract
Fumigation with phosphine gas is the primary method of controlling stored grain pests. In Turkey, phosphine has been used extensively since the 1950’s. Even though high levels of phosphine resistance have been detected in several key stored products pests across the world, it has never been studied in Turkey despite this long history of phosphine use. High-level phosphine resistance has been detected and genetically characterised previously in the rust red flour beetle, Tribolium castaneum in other countries. Since this pest is also a common problem in stored grain environment in Turkey, the current study was undertaken for the first time, to investigate the distribution and strength of phosphine resistance in T.castaneum. Four strains of T. castaneum were tested through bioassays for determining the weak and strong phosphine resistance phenotypes on the basis of the response of adults to discriminating phosphine concentrations of 0.03 mg/L and 0.25 mg/L, for 20 hour exposures respectively. Phenotype testing showed all strains exhibited some level of phosphine resistance with a maximum level of 196 fold. Sequencing and genetic testing of seven field-collected strains showed that all of them carried a strong resistance allele in at the rph2 locus similar to the one previously reported. Our results show that strong resistance to phosphine is common in Turkish strains of T. castaneum.
Keyword Dihydrolipoamide dehydrogenase
Dld
Molecular diagnostic
Phosphine resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
 
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