Bioanalytical method validation and quantification of flupirtine in canine plasma by HPLC with spectrofluorimetric detection

De Vito, Virginia, Saba, Alessandro, Owen, Helen and Giorgi, Mario (2015) Bioanalytical method validation and quantification of flupirtine in canine plasma by HPLC with spectrofluorimetric detection. American Journal of Animal and Veterinary Sciences, 10 2: 91-100. doi:10.3844/ajavsp.2015.91.100

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Author De Vito, Virginia
Saba, Alessandro
Owen, Helen
Giorgi, Mario
Title Bioanalytical method validation and quantification of flupirtine in canine plasma by HPLC with spectrofluorimetric detection
Journal name American Journal of Animal and Veterinary Sciences   Check publisher's open access policy
ISSN 1557-4563
1557-4555
Publication date 2015
Sub-type Article (original research)
DOI 10.3844/ajavsp.2015.91.100
Open Access Status DOI
Volume 10
Issue 2
Start page 91
End page 100
Total pages 10
Place of publication New York, NY, United States
Publisher Science Publications
Collection year 2016
Language eng
Formatted abstract
Flupirtine (FLU) is a non-opioid analgesic drug belongsto the class of N-Methyl-D-Aspartate (NMDA) antagonist without antipyretic orantiphlogistic properties. No analytical method to detect FLU in canine plasmasamples through a fluorimetric detector has been published to date. Theanalytical method described in this work provides a selective and accuratequantification of FLU. The mobile phase consisted of ACN:AcONH4 (20 mM) pH 6.8 (60:40, v/v) at a flow rate of 1 mL min-1 inisocratic mode. Excitation and emission wavelengths were set at 323 and 370 nm,respectively. The recoveries of FLU and IS (trazodone) were about 89 and 77%.Typical retention times for FLU and IS was 4.6±0.2 and 5.8±0.2 min,respectively. Limits of quantification and detection were 1 and 0.3 ng/mL,respectively. The described method was validated according to internationalguidelines on the bioanalytical method validation. The applicability of thismethod was verified by determining FLU in canine plasma after single oraltreatment with 5 mg kg-1 of Efiret®. The low LOQ showed that thepresent method could be useful for the FLU measurement even when administeredin sub-clinical doses.
Keyword Bioanalytical method
Dog
Flupirtine HPLC-FL
Plasma concentration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Veterinary Science Publications
 
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