Gestation related gene expression of the endocannabinoid pathway in rat placenta

Vaswani, Kanchan, Chan, Hsiu-Wen, Peiris, Hassendrini N., Nitert, Marloes Dekker, Wood Bradley, Ryan J., Armitage, James A., Rice, Gregory E. and Mitchell, Murray D. (2015) Gestation related gene expression of the endocannabinoid pathway in rat placenta. Mediators of Inflammation, 2015 1-9. doi:10.1155/2015/850471

Author Vaswani, Kanchan
Chan, Hsiu-Wen
Peiris, Hassendrini N.
Nitert, Marloes Dekker
Wood Bradley, Ryan J.
Armitage, James A.
Rice, Gregory E.
Mitchell, Murray D.
Title Gestation related gene expression of the endocannabinoid pathway in rat placenta
Journal name Mediators of Inflammation   Check publisher's open access policy
ISSN 1466-1861
Publication date 2015
Sub-type Article (original research)
DOI 10.1155/2015/850471
Open Access Status Not yet assessed
Volume 2015
Start page 1
End page 9
Total pages 9
Place of publication New York, NY, United States
Publisher Hindawi Publishing Corporation
Collection year 2016
Language eng
Formatted abstract
Mammalian placentation is a vital facet of the development of a healthy and viable offspring. Throughout gestation the placenta changes to accommodate, provide for, and meet the demands of a growing fetus. Gestational gene expression is a crucial part of placenta development. The endocannabinoid pathway is activated in the placenta and decidual tissues throughout pregnancy and aberrant endocannabinoid signaling during the period of placental development has been associated with pregnancy disorders. In this study, the gene expression of eight endocannabinoid system enzymes was investigated throughout gestation. Rat placentae were obtained at E14.25, E15.25, E17.25, and E20, RNA was extracted, and microarray was performed. Gene expression of enzymes Faah, Mgll, Plcd4, Pld1, Nat1, Daglα, and Ptgs2 was studied (cohort 1, microarray). Biological replication of the results was performed by qPCR (cohort 2). Four genes showed differential expression (Mgll, Plcd4, Ptgs2, and Pld1), from mid to late gestation. Genes positively associated with gestational age were Ptgs2, Mgll, and Pld1, while Plcd4 was downregulated. This is the first comprehensive study that has investigated endocannabinoid pathway gene expression during rat pregnancy. This study provides the framework for future studies that investigate the role of endocannabinoid system during pregnancy.
Keyword Gene expression
Endocannabinoid pathway
Mammalian placentation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2016 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 2 times in Scopus Article | Citations
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