Scattering of sculpted light in intact brain tissue, with implications for optogenetics

Favre-Bulle, Itia A., Preece, Daryl, Nieminen, Timo A., Heap, Lucy A., Scott, Ethan K. and Rubinsztein-Dunlop, Halina (2015) Scattering of sculpted light in intact brain tissue, with implications for optogenetics. Scientific Reports, 5 1-9. doi:10.1038/srep11501


Author Favre-Bulle, Itia A.
Preece, Daryl
Nieminen, Timo A.
Heap, Lucy A.
Scott, Ethan K.
Rubinsztein-Dunlop, Halina
Title Scattering of sculpted light in intact brain tissue, with implications for optogenetics
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015
Sub-type Article (original research)
DOI 10.1038/srep11501
Open Access Status DOI
Volume 5
Start page 1
End page 9
Total pages 9
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Abstract Optogenetics uses light to control and observe the activity of neurons, often using a focused laser beam. As brain tissue is a scattering medium, beams are distorted and spread with propagation through neural tissue, and the beam’s degradation has important implications in optogenetic experiments. To address this, we present an analysis of scattering and loss of intensity of focused laser beams at different depths within the brains of zebrafish larvae. Our experimental set-up uses a 488 nm laser and a spatial light modulator to focus a diffraction-limited spot of light within the brain. We use a combination of experimental measurements of back-scattered light in live larvae and computational modelling of the scattering to determine the spatial distribution of light. Modelling is performed using the Monte Carlo method, supported by generalised Lorenz–Mie theory in the single-scattering approximation. Scattering in areas rich in cell bodies is compared to that of regions of neuropil to identify the distinct and dramatic contributions that cell nuclei make to scattering. We demonstrate the feasibility of illuminating individual neurons, even in nucleus-rich areas, at depths beyond 100 μm using a spatial light modulator in combination with a standard laser and microscope optics.
Keyword Brain tissue
Optogenetics
Light scattering
Laser
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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