Roles of GBSSI and SSIIa in determining amylose fine structure

Wang, Kai, Hasjim, Jovin, Wu, Alex Chi, Li, Enpeng, Henry, Robert J. and Gilbert, Robert G. (2015) Roles of GBSSI and SSIIa in determining amylose fine structure. Carbohydrate Polymers, 127 264-274. doi:10.1016/j.carbpol.2015.03.081


Author Wang, Kai
Hasjim, Jovin
Wu, Alex Chi
Li, Enpeng
Henry, Robert J.
Gilbert, Robert G.
Title Roles of GBSSI and SSIIa in determining amylose fine structure
Journal name Carbohydrate Polymers   Check publisher's open access policy
ISSN 0144-8617
1879-1344
Publication date 2015-08-20
Sub-type Article (original research)
DOI 10.1016/j.carbpol.2015.03.081
Open Access Status
Volume 127
Start page 264
End page 274
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2016
Language eng
Formatted abstract
This study examines the relationships between genetics (single nucleotide polymorphisms (SNPs) in GBSSI and SSIIa genes), starch structure (amylose and amylopectin fine structures), and starch properties (relating to gelatinization). GBSSI and SSIIa SNPs did not alter the starch content of rice grains. GBSSI SNPs can affect the amylose content, but they are incapable of altering the chain-lengths of amylopectin and amylose. The amounts of both long and short amylose branches changed with the same trend as amylose content, and they appeared to affect starch gelatinization properties. SSIIa synthesizes intermediate single-lamella amylopectin chains (DP 16–21), and consequently impacts the gelatinization temperature. Mathematical modelling suggests that the reduction in SSIIa activity significantly increases the activity of SBEII, resulting in a decreased activity ratio of SS to SBE in the enzyme set governing an appropriate chain-length distribution range. This application of the genetics-structure-property paradigm provides selection strategies to produce rice varieties with improved qualities.
Keyword GBSSI
Biosynthesis
SSIIa
Single nucleotide polymorphisms
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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