Adhesion of microbes using 3-aminopropyl triethoxy silane and specimen stabilisation techniques for analytical transmission electron microscopy

Taylor, A.P., Webb, R.I., Barry, J.C., Hosmer, H., Gould, R.J. and Wood, B.J. (2000) Adhesion of microbes using 3-aminopropyl triethoxy silane and specimen stabilisation techniques for analytical transmission electron microscopy. Journal of Microscopy-oxford, 199 1: 56-67.


Author Taylor, A.P.
Webb, R.I.
Barry, J.C.
Hosmer, H.
Gould, R.J.
Wood, B.J.
Title Adhesion of microbes using 3-aminopropyl triethoxy silane and specimen stabilisation techniques for analytical transmission electron microscopy
Journal name Journal of Microscopy-oxford   Check publisher's open access policy
ISSN 0022-2720
1365-2818
Publication date 2000-07
Sub-type Article (original research)
DOI 10.1046/j.1365-2818.2000.00692.x
Volume 199
Issue 1
Start page 56
End page 67
Total pages 12
Place of publication Oxford
Publisher Blackwell Scientific Publications for the Royal Microscopical Society
Language eng
Subject C1
06 Biological Sciences
Abstract A variety of adhesive support-films were tested for their ability to adhere various biological specimens for transmission electron microscopy. Support films primed with 3-amino-propyl triethoxy silane (APTES), poly-L-lysine, carbon and ultraviolet-B (UV-B)-irradiated carbon were tested for their ability to adhere a variety of biological specimens including axenic cultures of Bacillus subtilis and Escherichia coli and wild-type magnetotactic bacteria. The effects of UV-B irradiation on the support film in the presence of air and electrostatic charge on primer deposition were tested and the stability of adhered specimens on various surfaces was also compared. APTES-primed UV-B-irradiated Pioloform(TM) was consistently the best adhesive, especially for large cells, and when adhered specimens were UV-B irradiated they became remarkably stable under an electron beam. This assisted the acquisition of in situ phase-contrast lattice images from a variety of biominerals in magnetotactic bacteria, in particular metastable greigite magnetosomes. Washing tests indicated that specimens adhering to APTES-primed UV-B-irradiated Pioloform(TM) were covalently coupled. The electron beam stability was hypothesised to be the result of mechanical strengthening of the specimen and support film and the reduced electrical resistance in the specimen and support film due to their polymerization and covalent coupling.
Keyword Microscopy
3-aminopropyl Triethoxy Silane
Adhesion
Aptes
Atem
Glow Discharge
Magnetosomes
Stabilization
Ultraviolet Irradiation
Treated Glass Slides
Magnetotactic Bacteria
Greigite Fe3s4
Crystal-growth
Morphology
Magnetite
Biomineralization
Polylysine
Magnetosomes
Particles
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Microscopy and Microanalysis Publications
 
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