Widespread prevalence of cryptic Symbiodinium D in the key Caribbean reef builder, Orbicella annularis

Kennedy, Emma V., Foster, Nicola L., Mumby, Peter J. and Stevens, Jamie R. (2015) Widespread prevalence of cryptic Symbiodinium D in the key Caribbean reef builder, Orbicella annularis. Coral Reefs, 34 2: 519-531. doi:10.1007/s00338-015-1264-4

Author Kennedy, Emma V.
Foster, Nicola L.
Mumby, Peter J.
Stevens, Jamie R.
Title Widespread prevalence of cryptic Symbiodinium D in the key Caribbean reef builder, Orbicella annularis
Journal name Coral Reefs   Check publisher's open access policy
ISSN 0722-4028
Publication date 2015
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s00338-015-1264-4
Open Access Status
Volume 34
Issue 2
Start page 519
End page 531
Total pages 13
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Symbiodinium D, a relatively rare clade of algal endosymbiont with a global distribution, has attracted interest as some of its sub-cladal types induce increased thermal tolerance and associated trade-offs, including reduced growth rate in its coral hosts. Members of Symbiodinium D are increasingly reported to comprise lowabundance ‘cryptic’ (\10 %) proportions of mixed coral endosymbiont communities, with unknown ecological implications.  Real-time PCR (RT-PCR) targeted to specific types is sufficiently sensitive to detect these background symbiont levels. In this study, RT-PCR was employed to screen 552 colonies of the key Caribbean reef builder Orbicella annularis sampled across a 5.4 million km2 range for the presence of cryptic Symbiodinium ‘D1’ (i.e., the principal Caribbean ITS2 variants, D1 and D1–4). All but one out of 33 populations analysed were shown to host low abundances of Symbiodinium D1, with an average of [30 % of corals per site found to harbour the symbiont.  When the same samples were analysed using the conventional screening technique, denaturing gradient gel electrophoresis, Symbiodinium D1 was only detected in 12 populations and appeared to be hosted by \12 % of colonies where present (in agreement with other reported low prevalence/absences in O. annularis). Cryptic Symbiodinium D1 showed a mainly uniform distribution across the wider Caribbean region, although significantly more Mesoamerican Barrier Reef corals hosted cryptic Symbiodinium D1 than might be expected by chance, possibly as a consequence of intense warming in the region in 1998.  Widespread prevalence of thermally tolerant Symbiodinium in O. annularis may potentially reflect a capacity for the coral to temporarily respond to warming events through symbiont shuffling. However, association with reduced coral calcification means that the ubiquitous nature of Symbiodinium D1 in O. annularis populations is unlikely to prevent long-term declines in reef health, at a time when maintaining reef growth is vital to sustain reef ecosystem function.
Keyword Zooxanthellae
Coral bleaching
Clade D
Climate change
Montastraea Int
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 13 Jul 2015, 09:55:57 EST by Ms May Balagaize on behalf of School of Biological Sciences