Fusion-protein-assisted protein crystallization

Kobe, Bostjan, Ve, Thomas and Williams, Simon J. (2015) Fusion-protein-assisted protein crystallization. Acta Crystallographica. Section F Structural Biology Communications, 71 7: 861-869. doi:10.1107/S2053230X15011061.

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Author Kobe, Bostjan
Ve, Thomas
Williams, Simon J.
Title Fusion-protein-assisted protein crystallization
Journal name Acta Crystallographica. Section F Structural Biology Communications   Check publisher's open access policy
ISSN 2053-230X
Publication date 2015-07-07
Year available 2015
Sub-type Article (original research)
DOI 10.1107/S2053230X15011061.
Open Access Status File (Publisher version)
Volume 71
Issue 7
Start page 861
End page 869
Total pages 9
Place of publication Malden, MA United States
Publisher Wiley-Blackwell Publishing
Collection year 2016
Language eng
Formatted abstract
Fusion proteins can be used directly in protein crystallization to assist crystallization in at least two different ways. In one approach, the `heterologous fusion-protein approach', the fusion partner can provide additional surface area to promote crystal contact formation. In another approach, the `fusion of interacting proteins approach', protein assemblies can be stabilized by covalently linking the interacting partners. The linker connecting the proteins plays different roles in the two applications: in the first approach a rigid linker is required to reduce conformational heterogeneity; in the second, conversely, a flexible linker is required that allows the native interaction between the fused proteins. The two approaches can also be combined. The recent applications of fusion-protein technology in protein crystallization from the work of our own and other laboratories are briefly reviewed.
Keyword Fusion of interacting proteins approach
Heterologous fusion protein approach
Linker
Membrane protein crystallization
Protein Interactions
Recombinant Fusion Protein
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes epub June 27 2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 10 Jul 2015, 09:23:30 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences