The depletion of global fossil fuel reserves and an increase in demand for fuel has led to the need to urgently research alternative sources of fuel to keep up with the high demand for energy. As there are limited reserves of fossil fuels which take millions of years to form, it is imperative that alternative and renewable sources of energy be developed rapidly. Biodiesel is an appropriate candidate as it has decreased emissions when compared to petro-diesel, food stocks are plentiful, and most importantly, it is renewable. However, there are many chemical and physical differences between the properties of petro-diesel and biodiesel. If biodiesel is to eventually replace petro-diesel, these differences are expected to provide various technical challenges to ensure that a standard diesel engine can operate cleanly andefficiently when fuelled with biodiesel. Currently, the operating parameters of the diesel engine are tailored to suit the use of petro-diesel as a fuel. Therefore optimizing the engines operating parameters for different specific fuel properties is essential. The following thesis consists of a literature review into how the injection parameters of a diesel engine can be tailored to lower emissions when biodiesel is used as a fuel. Numerous research papers on the topic were reviewed, and their experimental data compared and analysed in detail. It was found that both injection timing and injection pressure had a significant impact on the emissions of the engine as well other parameters such as fuel type, engine running conditions, and oxygen equivalence ratio. From this research, it was concluded that further testing and research into biofuels, and modifications to existing engines needs to be undertaken. With these recommendations in place it is thought that biodiesel can eventually completely replace petro-diesel as a lower emissions, renewable source of energy in the transportation, industrial and public sectors.