The Arabidopsis root stele transporter NPF2.3 contributes to nitrate translocation to shoots under salt stress

Taochy, Christelle, Gaillard, Isabelle, Ipotes, Emilie, Oomen, Ronald, Leonhardt, Nathalie, Zimmermann, Sabine, Peltier, Jean-Benoît, Szponarski, Wojciech, Simonneau, Thierry, Sentenac, Herve, Gibrat, Remy and Boyer, Jean-Christophe (2015) The Arabidopsis root stele transporter NPF2.3 contributes to nitrate translocation to shoots under salt stress. The Plant Journal, 83 3: 466-479. doi:10.1111/tpj.12901


Author Taochy, Christelle
Gaillard, Isabelle
Ipotes, Emilie
Oomen, Ronald
Leonhardt, Nathalie
Zimmermann, Sabine
Peltier, Jean-Benoît
Szponarski, Wojciech
Simonneau, Thierry
Sentenac, Herve
Gibrat, Remy
Boyer, Jean-Christophe
Title The Arabidopsis root stele transporter NPF2.3 contributes to nitrate translocation to shoots under salt stress
Journal name The Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2015-08
Sub-type Article (original research)
DOI 10.1111/tpj.12901
Volume 83
Issue 3
Start page 466
End page 479
Total pages 14
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2016
Language eng
Formatted abstract
In most plants, NO3- constitutes the major source of nitrogen, and its assimilation into amino acids is mainly achieved in shoots. Furthermore, recent reports have revealed that reduction of NO3- translocation from roots to shoots is involved in plant acclimation to abiotic stress. NPF2.3, a member of the NAXT (nitrate excretion transporter) sub-group of the NRT1/PTR family (NPF) from Arabidopsis, is expressed in root pericycle cells, where it is targeted to the plasma membrane. Transport assays using NPF2.3-enriched Lactococcus lactis membranes showed that this protein is endowed with NO3- transport activity, displaying a strong selectivity for NO3- against Cl-. In response to salt stress, NO3- translocation to shoots is reduced, at least partly because expression of the root stele NO3- transporter gene NPF7.3 is decreased. In contrast, NPF2.3 expression was maintained under these conditions. A loss-of-function mutation in NPF2.3 resulted in decreased root-to-shoot NO3- translocation and reduced shoot NO3- content in plants grown under salt stress. Also, the mutant displayed impaired shoot biomass production when plants were grown under mild salt stress. These mutant phenotypes were dependent on the presence of Na+ in the external medium. Our data indicate that NPF2.3 is a constitutively expressed transporter whose contribution to NO3- translocation to the shoots is quantitatively and physiologically significant under salinity.
Keyword Arabidopsis thaliana
Nitrate
Ion transporter
Root to shoot translocation
Salt stress
NPF family
Lactococcus lactis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 03 Jul 2015, 10:25:53 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences