Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions

Lim, SooA, Marcellin, Esteban, Jacob, Shana and Nielsen, Lars K. (2015) Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions. Journal of Proteomics, 126 24-33. doi:10.1016/j.jprot.2015.05.021

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Author Lim, SooA
Marcellin, Esteban
Jacob, Shana
Nielsen, Lars K.
Title Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions
Formatted title
Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1876-7737
1874-3919
Publication date 2015-08-03
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.jprot.2015.05.021
Open Access Status File (Author Post-print)
Volume 126
Start page 24
End page 33
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2016
Language eng
Abstract Little is known about the role of global phosphorylation events in the control of prokaryote metabolism. By performing a detailed analysis of all protein phosphorylation events previously reported in Escherichia coli, dynamic changes in protein phosphorylation were elucidated under three different culture conditions. Using scheduled reaction monitoring, the phosphorylation ratios of 82 peptides corresponding to 71 proteins were quantified to establish whether serine (S), threonine (T) and tyrosine (Y) phosphorylation events displayed a dynamic profile under changing culture conditions. The ratio of phosphorylation for 23 enzymes from central carbon metabolism was found to be dynamic. The data presented contributes to our understanding of the global role of phosphorylation in bacterial metabolism and highlight that phosphorylation is an important, yet poorly understood, regulatory mechanism of metabolism control in bacteria.
Keyword Central carbon metabolism
Phosphoproteomics
Phosphorylation
Quantitative phosphoproteomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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