Purification and Transcriptomic analysis of mouse fetal leydig cells reveals candidate genes for specification of gonadal steroidogenic cells

McClelland, Kathryn S., Bell, Katrina, Larney, Christian, Harley, Vincent R., Sinclair, Andrew H., Oshlack, Alicia, Koopman, Peter A. and Bowles, Josephine (2015) Purification and Transcriptomic analysis of mouse fetal leydig cells reveals candidate genes for specification of gonadal steroidogenic cells. Biology of Reproduction, 92 6: 145-145. doi:10.1095/​biolreprod.115.128918

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Author McClelland, Kathryn S.
Bell, Katrina
Larney, Christian
Harley, Vincent R.
Sinclair, Andrew H.
Oshlack, Alicia
Koopman, Peter A.
Bowles, Josephine
Title Purification and Transcriptomic analysis of mouse fetal leydig cells reveals candidate genes for specification of gonadal steroidogenic cells
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
1529-7268
Publication date 2015-06-01
Year available 2015
Sub-type Article (original research)
DOI 10.1095/​biolreprod.115.128918
Open Access Status
Volume 92
Issue 6
Start page 145
End page 145
Total pages 17
Place of publication Madison, WI United States
Publisher Society for the Study of Reproduction
Collection year 2016
Language eng
Formatted abstract
Male sex determination hinges on the development of testes in the embryo, beginning with the differentiation of Sertoli cells under the influence of the Y-linked gene SRY. Sertoli cells then orchestrate fetal testis formation including the specification of fetal Leydig cells (FLCs) that produce steroid hormones to direct virilization of the XY embryo. As the majority of XY disorders of sex development (DSDs) remain unexplained at the molecular genetic level, we reasoned that genes involved in FLC development might represent an unappreciated source of candidate XY DSD genes. To identify these genes, and to gain a more detailed understanding of the regulatory networks underpinning the specification and differentiation of the FLC population, we developed methods for isolating fetal Sertoli, Leydig, and interstitial cell-enriched subpopulations using an Sf1-eGFP transgenic mouse line. RNA sequencing followed by rigorous bioinformatic filtering identified 84 genes upregulated in FLCs, 704 genes upregulated in nonsteroidogenic interstitial cells, and 1217 genes upregulated in the Sertoli cells at 12.5 days postcoitum. The analysis revealed a trend for expression of components of neuroactive ligand interactions in FLCs and Sertoli cells and identified factors potentially involved in signaling between the Sertoli cells, FLCs, and interstitial cells. We identified 61 genes that were not known previously to be involved in specification or differentiation of FLCs. This dataset provides a platform for exploring the biology of FLCs and understanding the role of these cells in testicular development. In addition, it provides a basis for targeted studies designed to identify causes of idiopathic XY DSD.
Keyword Differentiation
Gonadogenesis
Leydig Cell
Neuroactive
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print 8/4/2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 26 Jun 2015, 15:08:07 EST by Susan Allen on behalf of Institute for Molecular Bioscience