Effects of electric fields on human mesenchymal stem cell behaviour and morphology using a novel multichannel device

Banks, T. A., Luckman, P. S. B., Frith, J. E. and Cooper-White, J. J. (2015) Effects of electric fields on human mesenchymal stem cell behaviour and morphology using a novel multichannel device. Integrative Biology (United Kingdom), 7 6: 693-712. doi:10.1039/c4ib00297k


Author Banks, T. A.
Luckman, P. S. B.
Frith, J. E.
Cooper-White, J. J.
Title Effects of electric fields on human mesenchymal stem cell behaviour and morphology using a novel multichannel device
Journal name Integrative Biology (United Kingdom)   Check publisher's open access policy
ISSN 1757-9708
1757-9694
Publication date 2015-06-01
Sub-type Article (original research)
DOI 10.1039/c4ib00297k
Open Access Status Not Open Access
Volume 7
Issue 6
Start page 693
End page 712
Total pages 20
Place of publication Cambridge, United Kingdom
Publisher R S C Publications
Collection year 2016
Language eng
Formatted abstract
The intrinsic piezoelectric nature of collagenous-rich tissues, such as bone and cartilage, can result in the production of small, endogenous electric fields (EFs) during applied mechanical stresses. In vivo, these EFs may influence cell migration, a vital component of wound healing. As a result, the application of small external EFs to bone fractures and cutaneous wounds is actively practiced clinically. Due to the significant regenerative potential of stem cells in bone and cartilage healing, and their potential role in the observed improved healing in vivo post applied EFs, using a novel medium throughput device, we investigated the impacts of physiological and aphysiological EFs on human bone marrow-derived mesenchymal stem cells (hBM-MSCs) for up to 15 hours. The applied EFs had significant impacts on hBM-MSC morphology and migration; cells displayed varying degrees of conversion to a highly elongated phenotype dependent on the EF strength, consistent perpendicular alignment to the EF vector, and definitive cathodal migration in response to EF strengths ≥0.5 V cm−1, with the fastest migration speeds observed at between 1.7 and 3 V cm−1. We observed variability in hBM-MSC donor-to-donor responses and overall tolerances to applied EFs. This study thus confirms hBM-MSCs are responsive to applied EFs, and their rate of migration towards the cathode is controllable depending on the EF strength, providing new insight into the physiology of hBM-MSCs and possibly a significant opportunity for the utilisation of EFs in directed scaffold colonisation in vitro for tissue engineering applications or in vivo post implantation.
Keyword Electric fields
Mesenchymal stem cells
Human bone marrow
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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