Purpurin Suppresses Candida albicans Biofilm Formation and Hyphal Development

Tsang, Paul Wai-Kei, Bandara, H. M. H. N. and Fong, Wing-Ping (2012) Purpurin Suppresses Candida albicans Biofilm Formation and Hyphal Development. PLoS One, 7 11: e50866-e50866. doi:10.1371/journal.pone.0050866


Author Tsang, Paul Wai-Kei
Bandara, H. M. H. N.
Fong, Wing-Ping
Title Purpurin Suppresses Candida albicans Biofilm Formation and Hyphal Development
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2012-11-30
Year available 2012
Sub-type Article (original research)
DOI 10.1371/journal.pone.0050866
Open Access Status DOI
Volume 7
Issue 11
Start page e50866
End page e50866
Total pages 8
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Abstract A striking and clinically relevant virulence trait of the human fungal pathogen Candida albicans is its ability to grow and switch reversibly among different morphological forms. Inhibition of yeast-to-hypha transition in C. albicans represents a new paradigm for antifungal intervention. We have previously demonstrated the novel antifungal activity of purpurin against Candida fungi. In this study, we extended our investigation by examining the in vitro effect of purpurin on C. albicans morphogenesis and biofilms. The susceptibility of C. albicans biofilms to purpurin was examined quantitatively by 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide reduction assay. Hyphal formation and biofilm ultrastructure were examined qualitatively by scanning electron microscopy (SEM). Quantitative reverse transcription-PCR (qRT-PCR) was used to evaluate the expression of hypha-specific genes and hyphal regulator in purpurin-treated fungal cells. The results showed that, at sub-lethal concentration (3 μg/ml), purpurin blocked the yeast-to-hypha transition under hypha-inducing conditions. Purpurin also inhibited C. albicans biofilm formation and reduced the metabolic activity of mature biofilms in a concentration-dependent manner. SEM images showed that purpurin-treated C. albicans biofilms were scanty and exclusively consisted of aggregates of blastospores. qRT-PCR analyses indicated that purpurin downregulated the expression of hypha-specific genes (ALS3, ECE1, HWP1, HYR1) and the hyphal regulator RAS1. The data strongly suggested that purpurin suppressed C. albicans morphogenesis and caused distorted biofilm formation. By virtue of the ability to block these two virulence traits in C. albicans, purpurin may represent a potential candidate that deserves further investigations in the development of antifungal strategies against this notorious human fungal pathogen in vivo.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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Created: Sun, 21 Jun 2015, 15:54:39 EST by Nihal Bandara on behalf of School of Dentistry