Improved biofilm antimicrobial activity of polyethylene glycol conjugated tobramycin compared to tobramycin in pseudomonas aeruginosa biofilms

Du, Ju, Bandara, H. M. H. N., Du, Ping, Huang, Hui, Hoang, Khang, Nguyen, Dang, Mogarala, Sri Vasudha and Smyth, Hugh D. C. (2015) Improved biofilm antimicrobial activity of polyethylene glycol conjugated tobramycin compared to tobramycin in pseudomonas aeruginosa biofilms. Molecular Pharmaceutics, 12 5: 1544-1553. doi:10.1021/mp500846u


Author Du, Ju
Bandara, H. M. H. N.
Du, Ping
Huang, Hui
Hoang, Khang
Nguyen, Dang
Mogarala, Sri Vasudha
Smyth, Hugh D. C.
Title Improved biofilm antimicrobial activity of polyethylene glycol conjugated tobramycin compared to tobramycin in pseudomonas aeruginosa biofilms
Journal name Molecular Pharmaceutics   Check publisher's open access policy
ISSN 1543-8392
1543-8384
Publication date 2015-05-04
Year available 2015
Sub-type Article (original research)
DOI 10.1021/mp500846u
Open Access Status
Volume 12
Issue 5
Start page 1544
End page 1553
Total pages 10
Place of publication Washington, DC United States
Publisher American Chemical Society
Collection year 2016
Language eng
Formatted abstract
The objective of this study was to develop a functionally enhanced antibiotic that would improve the therapeutic activity against bacterial biofilms. Tobramycin was chemically conjugated with polyethylene glycol (PEG) via site-specific conjugation to form PEGylated-tobramycin (Tob-PEG). The antibacterial efficacy of Tob-PEG, as compared to tobramycin, was assessed on the planktonic phase and biofilms phase of Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC80) of Tob-PEG was higher (13.9 μmol/L) than that of tobramycin (1.4 μmol/L) in the planktonic phases. In contrast, the Tob-PEG was approximately 3.2-fold more effective in eliminating bacterial biofilms than tobramycin. Specifically, Tob-PEG had a MIC80 lower than those exhibited by tobramycin (27.8 μmol/L vs 89.8 μmol/L). Both confocal laser scanning microscopy and scanning electron microscopy further confirmed these data. Thus, modification of antimicrobials by PEGylation appears to be a promising approach for overcoming the bacterial resistance in the established biofilms of Pseudomonas aeruginosa.
Keyword Cystic fibrosis
Minimum inhibitory concentration
Planktonic
Biofilm model
PEGylation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Dentistry Publications
 
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Created: Sun, 21 Jun 2015, 15:38:42 EST by Nihal Bandara on behalf of School of Dentistry