Exploration of peptides bound to MHC class I molecules in melanoma

Pritchard, Antonia L., Hastie, Marcus L., Neller, Michelle, Gorman, Jeffrey J., Schmidt, Chris W. and Hayward, Nicholas K. (2015) Exploration of peptides bound to MHC class I molecules in melanoma. Pigment Cell and Melanoma Research, 28 3: 281-294. doi:10.1111/pcmr.12357

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Author Pritchard, Antonia L.
Hastie, Marcus L.
Neller, Michelle
Gorman, Jeffrey J.
Schmidt, Chris W.
Hayward, Nicholas K.
Title Exploration of peptides bound to MHC class I molecules in melanoma
Journal name Pigment Cell and Melanoma Research   Check publisher's open access policy
ISSN 1755-148X
Publication date 2015-05
Sub-type Article (original research)
DOI 10.1111/pcmr.12357
Volume 28
Issue 3
Start page 281
End page 294
Total pages 14
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Collection year 2016
Language eng
Formatted abstract
Advancements in high-resolution HPLC and mass spectrometry have reinvigorated the application of this technology to identify peptides eluted from immunopurified MHC class I molecules. Three melanoma cell lines were assessed using w6/32 isolation, peptide elution and HPLC purification; peptides were identified by mass spectrometry. A total of 13 829 peptides were identified; 83–87% of these were 8–11 mers. Only approximately 15% have been described before. Subcellular locations of the source proteins showed even sampling; mRNA expression and total protein length were predictive of the number of peptides detected from a single protein. HLA-type binding prediction for 10 078 9/10 mer peptides assigned 88–95% to a patient-specific HLA subtype, revealing a disparity in strength of predicted binding. HLA-B*27-specific isolation successfully identified some peptides not found using w6/32. Sixty peptides were selected for immune screening, based on source protein and predicted HLA binding; no new peptides recognized by antimelanoma T cells were discovered. Additionally, mass spectrometry was unable to identify several epitopes targeted ex vivo by one patient's T cells.
Keyword Antigen
Mass spectrometry
MHC class I
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
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