Decellularized periodontal ligament cell sheets with recellularization potential

Farag, A., Vaquette, C., Theodoropoulos, C., Hamlet, S. M., Hutmacher, D. W. and Ivanovski, S. (2014) Decellularized periodontal ligament cell sheets with recellularization potential. Journal of Dental Research, 93 12: 1313-1319. doi:10.1177/0022034514547762

Author Farag, A.
Vaquette, C.
Theodoropoulos, C.
Hamlet, S. M.
Hutmacher, D. W.
Ivanovski, S.
Title Decellularized periodontal ligament cell sheets with recellularization potential
Journal name Journal of Dental Research   Check publisher's open access policy
ISSN 1544-0591
Publication date 2014-12-25
Sub-type Article (original research)
DOI 10.1177/0022034514547762
Open Access Status
Volume 93
Issue 12
Start page 1313
End page 1319
Total pages 7
Place of publication Thousand Oaks, CA, United States
Publisher SAGE Publications
Collection year 2015
Language eng
Formatted abstract
The periodontal ligament is the key tissue facilitating periodontal regeneration. This study aimed to fabricate decellularized human periodontal ligament cell sheets for subsequent periodontal tissue engineering applications. The decellularization protocol involved the transfer of intact human periodontal ligament cell sheets onto melt electrospun polycaprolactone membranes and subsequent bi-directional perfusion with NH4OH/Triton X-100 and DNase solutions. The protocol was shown to remove 92% of DNA content. The structural integrity of the decellularized cell sheets was confirmed by a collagen quantification assay, immunostaining of human collagen type I and fibronectin, and scanning electron microscopy. ELISA was used to demonstrate the presence of residual basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF) in the decellularized cell sheet constructs. The decellularized cell sheets were shown to have the ability to support recellularization by allogenic human periodontal ligament cells. This study describes the fabrication of decellularized periodontal ligament cell sheets that retain an intact extracellular matrix and resident growth factors and can support repopulation by allogenic cells. The decellularized hPDL cell sheet concept has the potential to be utilized in future “off-the-shelf” periodontal tissue engineering strategies.
Keyword Collagen
Extracellular matrix
Fibroblast growth factor
Tissue engineering
Vascular endothelial growth factor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
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Created: Thu, 18 Jun 2015, 22:52:45 EST by Amro Farag on behalf of Scholarly Communication and Digitisation Service