Characterization of intracellular translocation of Forkhead transcription factor O (FoxO) members induced by NGF in PC12 cells

Wen, Qiang, Duan, Xiaolu, Liao, Rifang, Little, Peter, Gao, Guoquan, Jiang, Hao, Lalit, Srivastava, Quirion, Remi and Zheng, Wenhua (2011) Characterization of intracellular translocation of Forkhead transcription factor O (FoxO) members induced by NGF in PC12 cells. Neuroscience Letters, 498 1: 31-36. doi:10.1016/j.neulet.2011.04.055


Author Wen, Qiang
Duan, Xiaolu
Liao, Rifang
Little, Peter
Gao, Guoquan
Jiang, Hao
Lalit, Srivastava
Quirion, Remi
Zheng, Wenhua
Title Characterization of intracellular translocation of Forkhead transcription factor O (FoxO) members induced by NGF in PC12 cells
Journal name Neuroscience Letters   Check publisher's open access policy
ISSN 0304-3940
1872-7972
Publication date 2011-07-01
Sub-type Article (original research)
DOI 10.1016/j.neulet.2011.04.055
Volume 498
Issue 1
Start page 31
End page 36
Total pages 6
Place of publication Shannon, Clare Ireland
Publisher Elsevier
Language eng
Abstract Nuclear translocation of Forkhead transcription factors of the O class (FoxOs) is important for the action of growth factors. However it is not known if all members of the FOXO family have the same translocation properties. We examined the effects of nerve growth factor (NGF) on nuclear/cytoplasmic shuttling of FoxO1, FoxO3a and FoxO6 in PC12 cells and determined their translocation kinetics. Our data demonstrated that NGF could induce the nuclear exclusion of FoxO1-GFP and FoxO3a-GFP in PC12 cells with different properties, but had no effect on FoxO6-GFP's nuclear localization and FoxO6-GFP showed an exclusive nuclear localization. Translocation of FoxO1 and FoxO3a could be blocked by K252a and LY294002 but not by PD98059.Moreover, FoxO3a returned to cytoplasm at a higher rate than FoxO1 after NGF stimulation and it was more sensitive than FoxO1 to NGF stimulation.
Keyword FoxO1
FoxO3a
Kinetics
NGF
PI3-K/Akt
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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