PAX6 does not regulate Nfia and Nfib expression during neocortical development

Bunt, Jens, Lim, Jonathan W., Zhao, Lu, Mason, Sharon and Richards, Linda J. (2015) PAX6 does not regulate Nfia and Nfib expression during neocortical development. Scientific Reports, 5 Art No.: 10668: . doi:10.1038/srep10668

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Author Bunt, Jens
Lim, Jonathan W.
Zhao, Lu
Mason, Sharon
Richards, Linda J.
Title PAX6 does not regulate Nfia and Nfib expression during neocortical development
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015
Year available 2015
Sub-type Article (original research)
DOI 10.1038/srep10668
Open Access Status DOI
Volume 5
Issue Art No.: 10668
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
The Nuclear factor I (NFI) family of transcription factors regulates proliferation and differentiation throughout the developing central nervous system. In the developing telencephalon of humans and mice, reduced Nfi expression is associated with agenesis of the corpus callosum and other neurodevelopmental defects. Currently, little is known about how Nfi expression is regulated during early telencephalic development. PAX6, a transcription factor important for telencephalic development, has been proposed as an upstream regulator of Nfi expression in the neocortex. Here we demonstrate that, in the developing neocortex of mice, NFIA and NFIB are endogenously expressed in gradients with high caudo-medial to low rostro-lateral expression and are most highly expressed in the cortical plate. We found that this expression pattern deviates from that of PAX6, suggesting that PAX6 does not drive Nfi expression. This is supported by in vitro reporter assays showing that PAX6 over-expression does not regulate Nfi promoter activity. Similarly, we also found that in the Pax6 Small Eye mutant, no changes in Nfi mRNA or protein expression are observed in the neocortical ventricular zone where PAX6 and the NFIs are expressed. Together these data demonstrate that in mice, PAX6 is not a transcriptional activator of Nfi expression during neocortical development.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2016 Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 2 times in Scopus Article | Citations
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Created: Fri, 12 Jun 2015, 15:27:38 EST by Susan Day on behalf of Queensland Brain Institute