A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients.

Goh, Lucas Y. H., Kam, Yiu-Wing, Metz, Stefan W., Hobson-Peters, Jody, Prow, Natalie A., McCarthy, Suzi, Smith, David W., Pijlman, Gorben P., Ng, Lisa F. and Hall, Roy A. (2015) A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients.. Journal of Virological Methods, 222 55-61. doi:10.1016/j.jviromet.2015.05.011


Author Goh, Lucas Y. H.
Kam, Yiu-Wing
Metz, Stefan W.
Hobson-Peters, Jody
Prow, Natalie A.
McCarthy, Suzi
Smith, David W.
Pijlman, Gorben P.
Ng, Lisa F.
Hall, Roy A.
Title A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients.
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 0166-0934
1879-0984
Publication date 2015-05
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.jviromet.2015.05.011
Open Access Status
Volume 222
Start page 55
End page 61
Total pages 7
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.
Keyword Chikungunya virus
Clinical diagnostics
Epitope blocking ELISA
Monoclonal antibodies
Virus like particles
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 05 Jun 2015, 09:36:32 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences