A genetic screen reveals a periplasmic copper chaperone required for nitrite reductase activity in pathogenic Neisseria.

Jen, Freda E. C., Djoko, Karrera Y., Bent, Stephen J., Day, Christopher J., McEwan, Alastair G. and Jennings, Michael P. (2015) A genetic screen reveals a periplasmic copper chaperone required for nitrite reductase activity in pathogenic Neisseria.. The FASEB Journal, 29 9: 3828-3838. doi:10.1096/fj.15-270751


Author Jen, Freda E. C.
Djoko, Karrera Y.
Bent, Stephen J.
Day, Christopher J.
McEwan, Alastair G.
Jennings, Michael P.
Title A genetic screen reveals a periplasmic copper chaperone required for nitrite reductase activity in pathogenic Neisseria.
Journal name The FASEB Journal   Check publisher's open access policy
ISSN 0892-6638
1530-6860
Publication date 2015-06
Year available 2015
Sub-type Article (original research)
DOI 10.1096/fj.15-270751
Open Access Status DOI
Volume 29
Issue 9
Start page 3828
End page 3838
Total pages 11
Place of publication Bethesda, MD, United States
Publisher Federation of American Societies for Experimental Biology
Collection year 2016
Language eng
Formatted abstract
Under conditions of low oxygen availability, Neisseria meningitidis and Neisseria gonorrhoeae are able to respire via a partial denitrification pathway in which nitrite is converted to nitrous oxide. In this process, nitrite reductase (AniA), a copper (Cu)-containing protein converts nitrite to NO, and this product is converted to nitrous oxide by nitric oxide reductase (NorB). NorB also confers protection against toxic NO, and so we devised a conditional lethal screen, using a norB mutant, to identify mutants that were resistant to nitrite-dependent killing. After random-deletion mutagenesis of N. meningitidis, this genetic screen identified a gene encoding a Cu chaperone that is essential for AniA function, AccA. Purified AccA binds one Cu (I) ion and also possesses a second binding site for Cu (II). This novel periplasmic Cu chaperone (AccA) appears to be essential for provision of Cu ions to AniA of pathogenic Neisseria to generate an active nitrite reductase. Apart from the Neisseria genus, AccA is distributed across a wide range of environmental Proteobacteria species.—Jen, F. E.-C., Djoko, K. Y., Bent, S. J., Day, C. J., McEwan, A. G., Jennings, M. P. A genetic screen reveals a periplasmic copper chaperone required for nitrite reductase activity in pathogenic Neisseria.
Keyword Meningitis
Gonorrhea
Denitrification
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 05 Jun 2015, 09:32:20 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences