Cardiac gene expression data and in silico analysis provide novel insights into human and mouse taste receptor gene regulation

Foster, Simon R., Porrello, Enzo R., Stefani, Maurizio, Smith, Nicola J., Molenaar, Peter, Dos Remedios, Cristobal G., Thomas, Walter G. and Ramialison, Mirana (2015) Cardiac gene expression data and in silico analysis provide novel insights into human and mouse taste receptor gene regulation. Naunyn-Schmiedeberg's Archives of Pharmacology, 388 10: 1009-1027. doi:10.1007/s00210-015-1118-1


Author Foster, Simon R.
Porrello, Enzo R.
Stefani, Maurizio
Smith, Nicola J.
Molenaar, Peter
Dos Remedios, Cristobal G.
Thomas, Walter G.
Ramialison, Mirana
Title Cardiac gene expression data and in silico analysis provide novel insights into human and mouse taste receptor gene regulation
Journal name Naunyn-Schmiedeberg's Archives of Pharmacology   Check publisher's open access policy
ISSN 1432-1912
0028-1298
Publication date 2015-05
Sub-type Article (original research)
DOI 10.1007/s00210-015-1118-1
Open Access Status Not yet assessed
Volume 388
Issue 10
Start page 1009
End page 1027
Total pages 19
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2016
Language eng
Abstract G protein-coupled receptors are the principal mediators of the sweet, umami, bitter, and fat taste qualities in mammals. Intriguingly, the taste receptors are also expressed outside of the oral cavity, including in the gut, airways, brain, and heart, where they have additional functions and contribute to disease. However, there is little known about the mechanisms governing the transcriptional regulation of taste receptor genes. Following our recent delineation of taste receptors in the heart, we investigated the genomic loci encoding for taste receptors to gain insight into the regulatory mechanisms that drive their expression in the heart. Gene expression analyses of healthy and diseased human and mouse hearts showed coordinated expression for a subset of chromosomally clustered taste receptors. This chromosomal clustering mirrored the cardiac expression profile, suggesting that a common gene regulatory block may control the taste receptor locus. We identified unique domains with strong regulatory potential in the vicinity of taste receptor genes. We also performed de novo motif enrichment in the proximal promoter regions and found several overrepresented DNA motifs in cardiac taste receptor gene promoters corresponding to ubiquitous and cardiac-specific transcription factor binding sites. Thus, combining cardiac gene expression data with bioinformatic analyses, this study has provided insights into the noncoding regulatory landscape for taste GPCRs. These findings also have broader relevance for the study of taste GPCRs outside of the classical gustatory system, where understanding the mechanisms controlling the expression of these receptors may have implications for future therapeutic development.
Keyword Cis-regulatory modules
De novo motif discovery
Heart
Taste receptor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biomedical Sciences Publications
School of Medicine Publications
 
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