Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy

Nagel, Robyn, Gray, Christian, Bielefeldt-Ohmann, Helle and Traub, Rebecca J. (2015) Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy. Parasitology Research, 114 9: 3237-3245. doi:10.1007/s00436-015-4540-x


Author Nagel, Robyn
Gray, Christian
Bielefeldt-Ohmann, Helle
Traub, Rebecca J.
Title Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy
Formatted title
Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy
Journal name Parasitology Research   Check publisher's open access policy
ISSN 1432-1955
0932-0113
Publication date 2015-05-22
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s00436-015-4540-x
Open Access Status DOI
Volume 114
Issue 9
Start page 3237
End page 3245
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2016
Language eng
Formatted abstract
Blastocystis spp. are common human enteric parasites with complex morphology and have been reported to cause irritable bowel syndrome (IBS). Deconvolutional microscopy with time-lapse imaging and fluorescent spectroscopy of xenic cultures of Blastocystis spp. from stool samples of IBS patients and from asymptomatic, healthy pigs allowed observations of living organisms in their natural microbial environment. Blastocystis organisms of the vacuolated, granular, amoebic and cystic forms were observed to autofluorescence in the 557/576 emission spectra. Autofluorescence could be distinguished from fluorescein-conjugated Blastocystis-specific antibody labelling in vacuolated and granular forms. This antibody labelled Blastocystis subtypes 1, 3 and 4 but not 5. Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h. Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation. Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.
Keyword Autofluorescence
Blastocystis
Deconvolutional microscopy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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