Time-dependent expression and activity of cytochrome P450 1s in early life-stages of the zebrafish (Danio rerio)

Braunig, Jennifer, Schiwy, Sabrina, Broedel, Oliver, Muller, Yvonne, Frohme, Marcus, Hollert, Henner and Keiter, Steffen H (2015) Time-dependent expression and activity of cytochrome P450 1s in early life-stages of the zebrafish (Danio rerio). Environmental Science and Pollution Research, 22 21: 16319-16328. doi:10.1007/s11356-015-4673-6


Author Braunig, Jennifer
Schiwy, Sabrina
Broedel, Oliver
Muller, Yvonne
Frohme, Marcus
Hollert, Henner
Keiter, Steffen H
Title Time-dependent expression and activity of cytochrome P450 1s in early life-stages of the zebrafish (Danio rerio)
Journal name Environmental Science and Pollution Research   Check publisher's open access policy
ISSN 1614-7499
0944-1344
Publication date 2015-05-22
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s11356-015-4673-6
Open Access Status Not yet assessed
Volume 22
Issue 21
Start page 16319
End page 16328
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2016
Language eng
Formatted abstract
Zebrafish embryos are being increasingly used as model organisms for the assessment of single substances and complex environmental samples for regulatory purposes. Thus, it is essential to fully understand the xenobiotic metabolism during the different life-stages of early development. The aim of the present study was to determine arylhydrocarbon receptor (AhR)-mediated activity during selected times of early development using qPCR, enzymatic activity through measurement of 7-ethoxyresorufin-O-deethylase (EROD) activity, and protein expression analysis. In the present study, gene expression of cyp1a, cyp1b1, cyp1c1, cyp1c2, and ahr2 as well as EROD activity were investigated up to 120 h postfertilization (hpf) after exposure to either β-naphthoflavone (BNF) or a polycyclic aromatic hydrocarbons (PAH)-contaminated sediment extract from Vering Kanal in Hamburg (VK). Protein expression was measured at 72 hpf after exposure to 20 μg/L BNF. Altered proteins were identified by matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) peptide mass fingerprinting. Distinct patterns of basal messenger RNA (mRNA) expression were found for each of the cyp1 genes, suggesting specific roles during embryonic development. All transcripts were induced by BNF and VK. ahr2 mRNA expression was significantly upregulated after exposure to VK. All cyp1 genes investigated showed a temporal decline in expression at 72 hpf. The significant decline of Hsp 90β protein at 72 hpf after exposure to BNF may suggest an explanation for the decline of cyp1 genes at this time point as Hsp 90β is of major importance for the functioning of the Ah-receptor. EROD activity measured in embryos was significantly induced after 96 hpf of exposure to BNF or VK. Together, these results demonstrate distinct temporal patterns of cyp1 genes and protein activities in zebrafish embryos as well as show a need to investigate further the xenobiotic biotransformation system during early development of zebrafish.
Keyword Arylhydrocarbon receptor
β-naphthoflavone
Cytochrome P450
EROD
Hsp 90β
Zebrafish
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
National Research Centre for Environmental Toxicology Publications
 
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