Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels

Lopez-Sanchez, Patricia, Schuster, Erich, Wang, Dongjie, Gidley, Michael J. and Strom, Anna (2015) Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels. Soft Matter, 11 20: 4002-4010. doi:10.1039/c5sm00103j


Author Lopez-Sanchez, Patricia
Schuster, Erich
Wang, Dongjie
Gidley, Michael J.
Strom, Anna
Title Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
Journal name Soft Matter   Check publisher's open access policy
ISSN 1744-6848
1744-683X
Publication date 2015-05-28
Year available 2015
Sub-type Article (original research)
DOI 10.1039/c5sm00103j
Open Access Status Not Open Access
Volume 11
Issue 20
Start page 4002
End page 4010
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2016
Language eng
Formatted abstract
Cellulose hydrogels are extensively applied in many biotechnological fields and are also used as models for plant cell walls. We synthesised model cellulosic hydrogels containing hemicelluloses, as a biomimetic of plant cell walls, in order to study the role of hemicelluloses on their mass transport properties. Microbial cellulose is able to self-assemble into composites when hemicelluloses, such as xyloglucan and arabinoxylan, are present in the incubation media, leading to hydrogels with different nano and microstructures. We investigated the diffusivities of a series of fluorescently labelled dextrans, of different molecular weight, and proteins, including a plant pectin methyl esterase (PME), using fluorescence recovery after photobleaching (FRAP). The presence of xyloglucan, known to be able to crosslink cellulose fibres, confirmed by scanning electron microscopy (SEM) and 13C NMR, reduced mobility of macromolecules of molecular weight higher than 10 kDa, reflected in lower diffusion coefficients. Furthermore PME diffusion was reduced in composites containing xyloglucan, despite the lack of a particular binding motif in PME for this polysaccharide, suggesting possible non-specific interactions between PME and this hemicellulose. In contrast, hydrogels containing arabinoxylan coating cellulose fibres showed enhanced diffusivity of the molecules studied. The different diffusivities were related to the architectural features found in the composites as a function of polysaccharide composition. Our results show the effect of model hemicelluloses in the mass transport properties of cellulose networks in highly hydrated environments relevant to understanding the role of hemicelluloses in the permeability of plant cell walls and aiding design of plant based materials with tailored properties.
Keyword Plant cell walls
Biomimetics
Diffusion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Centre for Nutrition and Food Sciences Publications
 
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