Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.)

Pang, Wenxing, Li, Xiaonan, Choi, Su Ryun, Nguyen, Van Dan, Dhandapani, Vignesh, Kim, Yoon-Young, Ramchiary, Nirala, Kim, Jin Gyu, Edwards, David, Batley, Jacqueline, Na, Jonghyun, Kim, HyeRan and Lim, Yong Pyo (2015) Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.). Molecular Breeding, 35 5: 1-12. doi:10.1007/s11032-015-0318-1


Author Pang, Wenxing
Li, Xiaonan
Choi, Su Ryun
Nguyen, Van Dan
Dhandapani, Vignesh
Kim, Yoon-Young
Ramchiary, Nirala
Kim, Jin Gyu
Edwards, David
Batley, Jacqueline
Na, Jonghyun
Kim, HyeRan
Lim, Yong Pyo
Title Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.)
Formatted title
Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.)
Journal name Molecular Breeding   Check publisher's open access policy
ISSN 1380-3743
1572-9788
Publication date 2015-05
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s11032-015-0318-1
Open Access Status Not yet assessed
Volume 35
Issue 5
Start page 1
End page 12
Total pages 12
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2016
Language eng
Formatted abstract
Cabbage head splitting can greatly affect both the quality and commercial value of cabbage (Brassica oleracea). To detect the genetic basis of head-splitting resistance, a genetic map was constructed using an F2 population derived by crossing “748” (head-splitting-resistant inbred line) and “747” (head-splitting-susceptible inbred line). The map spans 830.9 cM and comprises 270 markers distributed in nine linkage groups, which correspond to the nine chromosomes of B. oleracea. The average distance between adjacent markers was 3.6 cM. A total of six quantitative trait loci (QTLs) conferring resistance to head splitting were detected in chromosome 2, 4, and 6. Two QTLs, SPL-2-1 and SPL-4-1, on chromosomes 2 and 4, respectively, were detected in the experiments over 2 years, suggesting that these two potential loci were important for governing the head-splitting resistance trait. Markers BRPGM0676 and BRMS137, which were tightly linked with head-splitting resistance, were detected in the conserved QTL SPL-2-1 region using bulked segregant analysis. Synteny analysis showed that SPL-2-1 was anchored to a 3.18-Mb genomic region of the B. oleracea genome, homologous to crucifer ancestral karyotype E block in chromosome 1 of Arabidopsis thaliana. Moreover, using a field emission scanning electron microscope, significant differences were observed between the two parental lines in terms of cell structures. Line “747” had thinner cell wall, lower cell density, larger cell size, and anomalous cell wall structure compared with the resistant line “748.” The different cell structures can provide a cytological base for assessing cabbage head splitting.
Keyword Cabbage
Head-splitting resistance
Quantitative trait loci
Bulked segregant analysis
Genetic map
Scanning electron microscope
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 26 May 2015, 00:35:43 EST by System User on behalf of School of Agriculture and Food Sciences