Ciliates from ancient permafrost: assessment of cold resistance of the resting cysts

Shatilovich, Anastasia, Stoupin, Daniel and Rivkina, Elizaveta (2015) Ciliates from ancient permafrost: assessment of cold resistance of the resting cysts. European Journal of Protistology, 51 3: 230-240. doi:10.1016/j.ejop.2015.04.001


Author Shatilovich, Anastasia
Stoupin, Daniel
Rivkina, Elizaveta
Title Ciliates from ancient permafrost: assessment of cold resistance of the resting cysts
Journal name European Journal of Protistology   Check publisher's open access policy
ISSN 1618-0429
0932-4739
Publication date 2015-06-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.ejop.2015.04.001
Open Access Status
Volume 51
Issue 3
Start page 230
End page 240
Total pages 11
Place of publication Elsevier
Publisher Amsterdam, Netherlands
Collection year 2016
Language eng
Formatted abstract
There is evidence that resting cysts of soil ciliates and numerous taxa of other protists can survive in permafrost for thousands of years at subzero temperatures; however, our knowledge about mechanisms of long term cryobiosis remains incomplete. In order to better understand the means by which ancient cysts survive, we investigated resistance to cyclical supercooling stress of resting cysts of the soil ciliate Colpoda steinii (Colpodida, Ciliophora). Three clonal strains were used for comparison, isolated from Siberian tundra soil, ancient Holocene (5–7,000 y) and late Pleistocene (32–35,000 y) permafrost sediments. To determine the viability of the ancient and contemporary ciliate cysts we improved and validated a cultivation-independent method of vital fluorescent staining with a combination of two nucleic acid binding dyes, acridine orange and propidium iodide. The viability of Colpoda steinii cysts during low-temperature experiments was measured using both the proposed vital fluorescent staining method and standard germination test. Our results indicate that the dual-fluorescence technique is a more accurate, rapid, and efficient method for estimating cyst viability. We found that cysts of ancient ciliates display lower tolerance to the impact of cyclical cold compared to cysts of contemporary ciliates from Siberian permafrost affected soils.
Keyword Colpoda steinii
Permafrost
Viability
Fluorogenic dyes
Excystation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
 
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