Evolutionary engineering improves tolerance for replacement jet fuels in Saccharomyces cerevisiae

Brennan, Timothy C. R., Williams, Thomas C., Schulz, Benjamin L., Palfreyman, Robin W., Kroemer, Jens O. and Nielsen, Lars K. (2015) Evolutionary engineering improves tolerance for replacement jet fuels in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 81 10: 3316-3325. doi:10.1128/AEM.04144-14

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Author Brennan, Timothy C. R.
Williams, Thomas C.
Schulz, Benjamin L.
Palfreyman, Robin W.
Kroemer, Jens O.
Nielsen, Lars K.
Title Evolutionary engineering improves tolerance for replacement jet fuels in Saccharomyces cerevisiae
Formatted title
Evolutionary engineering improves tolerance for replacement jet fuels in Saccharomyces cerevisiae
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2015-05
Year available 2015
Sub-type Article (original research)
DOI 10.1128/AEM.04144-14
Open Access Status File (Publisher version)
Volume 81
Issue 10
Start page 3316
End page 3325
Total pages 10
Place of publication Washington, DC United States
Publisher American Society for Microbiology
Collection year 2016
Language eng
Formatted abstract
Monoterpenes are liquid hydrocarbons with applications ranging from flavor and fragrance to replacement jet fuel. Their toxicity, however, presents a major challenge for microbial synthesis. Here we evolved limonene-tolerant Saccharomyces cerevisiae strains and sequenced six strains across the 200-generation evolutionary time course. Mutations were found in the tricalbin proteins Tcb2p and Tcb3p. Genomic reconstruction in the parent strain showed that truncation of a single protein (tTcb3p1-989), but not its complete deletion, was sufficient to recover the evolved phenotype improving limonene fitness 9-fold. tTcb3p1-989 increased tolerance toward two other monoterpenes (β-pinene and myrcene) 11- and 8-fold, respectively, and tolerance toward the biojet fuel blend AMJ-700t (10% cymene, 50% limonene, 40% farnesene) 4-fold. tTcb3p1-989 is the first example of successful engineering of phase tolerance and creates opportunities for production of the highly toxic C10 alkenes in yeast.
Keyword Cell wall
Plasma membrane
Monoterpene
Proteins
Genome
Identification
Glycosylation
Glycoproteins
Biofuels
Biology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/81/10/3316/suppl/DCSupplemental

 
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