Development of P2 olfactory glomeruli in P2-internal ribosome entry site-tau-lacZ transgenic mice

Royal, Stephanie J. and Key, Brian (1999) Development of P2 olfactory glomeruli in P2-internal ribosome entry site-tau-lacZ transgenic mice. Journal of Neuroscience, 19 22: 9856-9864.

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Author Royal, Stephanie J.
Key, Brian
Title Development of P2 olfactory glomeruli in P2-internal ribosome entry site-tau-lacZ transgenic mice
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 0270-6474
Publication date 1999-11
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 19
Issue 22
Start page 9856
End page 9864
Total pages 9
Editor Gordon M. Shepherd
Place of publication Washington DC
Publisher Society of Neuroscience
Language eng
Subject C1
06 Biological Sciences
060410 Neurogenetics
Abstract Primary olfactory neurons project their axons to the olfactory bulb, where they terminate in discrete loci called glomeruli. All neurons expressing the same odorant receptor appear to terminate in a few glomeruli in each olfactory bulb. In the P2-IRES-tau-LacZ line of transgenic mice, LacZ is expressed in the perikarya and axons of primary olfactory neurons that express the P2 odorant receptor. In the present study, we examined the developmental appearance of P2 neurons, the topographical targeting of P2 axons, as well as the formation of P2 glomeruli in the olfactory bulb. P2 axons were first detected in the olfactory nerve fiber layer at embryonic day 14.5 (E14.5), and by E15.5 these axons terminated in a broad locus in the presumptive glomerular layer. During the next 5 embryonic days, the elongated cluster of axons developed into discrete glomerulus-like structures. In many cases, glomeruli appeared as pairs, which were initially connected by a fascicle of P2 axons. This connection was lost by postnatal day 7.5, and double glomeruli at the same locus were observed in 85% of adult animals. During the early postnatal period, there was considerable mistargeting of P2 axons. In some cases P2 axons entered inappropriate glomeruli or continued to grow past the glomerular layer into the deeper layers of the olfactory bulb. These aberrant axons were not observed in adult animals. These results indicate that olfactory axons exhibit errors while converging onto a specific glomerulus and suggest that guidance cues may be diffusely distributed at target sites in the olfactory bulb.
Keyword Neurosciences
P2 Receptor
Odorant Receptor Expression
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 126 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 13 Aug 2007, 11:27:27 EST