Charge and interfacial behavior of short side-chain heavily glycosylated porcine stomach mucin

Yakubov, Gleb E., Papagiannopoulos, Aristeidis, Rat, Elodie and Waigh, Thomas A. (2007) Charge and interfacial behavior of short side-chain heavily glycosylated porcine stomach mucin. Biomacromolecules, 8 12: 3791-3799. doi:10.1021/bm700721c


Author Yakubov, Gleb E.
Papagiannopoulos, Aristeidis
Rat, Elodie
Waigh, Thomas A.
Title Charge and interfacial behavior of short side-chain heavily glycosylated porcine stomach mucin
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1021/bm700721c
Volume 8
Issue 12
Start page 3791
End page 3799
Total pages 9
Language eng
Subject 1605 Policy and Administration
1300 Biochemistry, Genetics and Molecular Biology
2507 Polymers and Plastics
2505 Materials Chemistry
Abstract The current accepted model for high-molecular-weight gastric mucins of the MUC family is that they adopt a polydisperse coil conformation in bulk solutions. We develop this model using well-characterized highly purified porcine gastric mucin and examine the molecules' charge and interfacial adsorption. "Orthana" mucin has short side-chains, low levels of sialic acid residues, and includes minute amounts of cystine residues that can be responsible for the self-polymerization of mucin. Atomic force microscopy and transmission electron microscopy are used to examine the interfacial behavior of the mucin and clearly demonstrate the existence of discrete spherical subunits within the mucin molecules, with sizes in agreement with static light scattering, dynamic light scattering, and ζ potential measurements. Furthermore images indicate the combs are assembled with a beads on a string conformation; the daisy chain model. Zeta potential measurements establish the polyampholyte nature of the mucin molecules, which is used to explain their adsorption behavior on similarly charged surfaces.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Citation counts: TR Web of Science Citation Count  Cited 34 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
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Created: Fri, 15 May 2015, 02:51:03 EST by Gleb Yakubov