Mitochondrial displacements in response to nanomechanical forces

Silberberg, Yaron R., Pelling, Andrew E., Yakubov, Gleb E., Crum, William R., Hawkes, David J. and Horton, Mike A. (2008) Mitochondrial displacements in response to nanomechanical forces. Journal of Molecular Recognition, 21 1: 30-36. doi:10.1002/jmr.868

Author Silberberg, Yaron R.
Pelling, Andrew E.
Yakubov, Gleb E.
Crum, William R.
Hawkes, David J.
Horton, Mike A.
Title Mitochondrial displacements in response to nanomechanical forces
Journal name Journal of Molecular Recognition   Check publisher's open access policy
ISSN 0952-3499
Publication date 2008
Sub-type Article (original research)
DOI 10.1002/jmr.868
Volume 21
Issue 1
Start page 30
End page 36
Total pages 7
Language eng
Subject 1303 Specialist Studies in Education
1311 Genetics
1707 Computer Vision and Pattern Recognition
2403 Immunology
1312 Molecular Biology
Abstract Mechanical stress affects and regulates many aspects of the cell, including morphology, growth, differentiation, gene expression and apoptosis. In this study we show how mechanical stress perturbs the intracellular structures of the cell and induces mechanical responses. In order to correlate mechanical perturbations to cellular responses, we used a combined fluorescence-atomic force microscope (AFM) to produce well defined nanomechanical perturbations of 10 nN while simultaneously tracking the real-time motion of fluorescently labelled mitochondria in live cells. The spatial displacement of the organelles in response to applied loads demonstrates the highly dynamic mechanical response of mitochondria in fibroblast cells. The average displacement of all mitochondrial structures analysed showed an increase of ∼40%, post-perturbation (∼160 nm in comparison to basal displacements of ∼110 nm). These results show that local forces can produce organelle displacements at locations far from the initial point of contact (up to ∼40 μm). In order to examine the role of the cytoskeleton in force transmission and its effect on mitochondrial displacements, both the actin and microtubule cytoskeleton were disrupted using Cytochalasin D and Nocodazole, respectively. Our results show that there is no significant change in mitochondrial displacement following indentation after such treatments. These results demonstrate the role of the cytoskeleton in force transmission through the cell and on mitochondrial displacements. In addition, it is suggested that care must be taken when performing mechanical experiments on living cells with the AFM, as these local mechanical perturbations may have significant structural and even biochemical effects on the cell. Copyright
Keyword Atomic force microscopy
Fluorescence microscopy
Particle tracking
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
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Created: Thu, 14 May 2015, 16:43:10 EST by Gleb Yakubov