Polyphenol control of cell spreading on glycoprotein substrata

McColl, James, Horvath, Robert, Aref, Amirreza, Larcombe, Lee, Chianella, Iva, Morgan, Sarah, Yakubov, Gleb E. and Ramsden, Jeremy J. (2009) Polyphenol control of cell spreading on glycoprotein substrata. Journal of Biomaterials Science, Polymer Edition, 20 5-6: 841-851. doi:10.1163/156856209X427023


Author McColl, James
Horvath, Robert
Aref, Amirreza
Larcombe, Lee
Chianella, Iva
Morgan, Sarah
Yakubov, Gleb E.
Ramsden, Jeremy J.
Title Polyphenol control of cell spreading on glycoprotein substrata
Journal name Journal of Biomaterials Science, Polymer Edition   Check publisher's open access policy
ISSN 0920-5063
1568-5624
Publication date 2009-03-01
Year available 2009
Sub-type Article (original research)
DOI 10.1163/156856209X427023
Open Access Status
Volume 20
Issue 5-6
Start page 841
End page 851
Total pages 11
Place of publication Abingdon, Oxfordshire, United Kingdom
Publisher Taylor and Francis
Language eng
Abstract Cell-surface contacts are vital for many eukaryotic cells. The surface provides anchorage (facilitating spreading and proliferation), is involved in sensation, i.e., via mechano-, osmo- and chemoreceptors, and in addition nutrients may also be supplied via vessels adjacent to the basal lamina. Hence, the ability to manipulate the surface characteristics provides a mechanism for directly influencing cell behaviour. Applications such as medical implants and tissue engineering require biocompatible, stable surfaces for controlling cell behaviour. Mucin-coated surfaces inhibit cell spreading compared with poly(L-lysine) in vitro; here, we show that a composite layer assembled from mucin-EGCg aggregates counters the inhibition. Although the anti-spreading effects of the glycoprotein substratum on cell behaviour are similar to those observed for pure polysaccharide surfaces, the reversal of cell spreading inhibition by the admixture of polyphenol/glycoprotein substrata is remarkable and unexpected. Possible applications for a composite glycoprotein-polyphenol layer include medical devices, in particular for those operating at mucosal interfaces such as the oral, tracheal or gastrointestinal tract cavities, wound healing, cancer control and the controlled growth of therapeutic cell cultures.
Keyword Cell spreading
Confocal miscroscopy
Dynamic light scattering
Egcg
Mucin
Optical waveguide lightmode spectroscopy
Viscoelasticity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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Created: Thu, 14 May 2015, 16:39:13 EST by Gleb Yakubov on behalf of School of Chemical Engineering