Free radical scavengers improve liver function but not morphological changes induced by reperfusion injury

Arab, Hossein-Ali, Walker, Neal I., Cheung, Kee, Hickman, Peter E., Potter, Jolia M., Kadkhodaee, Mehri and Roberts, Michael S. (2015) Free radical scavengers improve liver function but not morphological changes induced by reperfusion injury. Journal of Investigative Surgery, 28 2: 77-85. doi:10.3109/08941939.2014.971205


Author Arab, Hossein-Ali
Walker, Neal I.
Cheung, Kee
Hickman, Peter E.
Potter, Jolia M.
Kadkhodaee, Mehri
Roberts, Michael S.
Title Free radical scavengers improve liver function but not morphological changes induced by reperfusion injury
Journal name Journal of Investigative Surgery   Check publisher's open access policy
ISSN 1521-0553
0894-1939
Publication date 2015-04
Sub-type Article (original research)
DOI 10.3109/08941939.2014.971205
Volume 28
Issue 2
Start page 77
End page 85
Total pages 9
Place of publication London, United Kingdom
Publisher Informa Healthcare
Collection year 2016
Language eng
Formatted abstract
Objective:  Reperfusion injury (RI) is associated with high generation of reactive oxygen species (ROS), but the extent of involvement of these agents in the injury remains controversial. The present study aimed to examine the effectiveness of ROS scavengers against hepatic reperfusion injury in the rat.

Methods:  The RI was induced in the liver using an isolated slow-flow, reflow perfused rat liver in both anterograde and retrograde perfusion. The effects of gentisic acid, N-acetyl cysteine, and trolox C on the superoxide production, liver function, and morphological changes were examined using different biochemical and histological assays.

Results:  The hepatic RI caused a significant (p < 0.05) increase in superoxide production and enzyme releases and a decrease in bile flow in both directions. Histological changes induced by RI include apoptosis, necrosis, pale cytoplasm, cell vacuolation, and attenuation of cell cords. Although the production of superoxide in retrograde direction was significantly less than the anterograde, the extent of the injury in the retrograde was greater than the anterograde direction. Pretreatment of the livers with each of the test compounds significantly reduced the release of lactate dehydrogenase and aspartate aminotransferase and improved bile flow in the liver exposed to hypoxia/reperfusion. However, they failed to protect the liver against the structural alterations induced by RI.

Conclusion:  ROS scavengers can reduce superoxide-induced damage and improve the liver function, but they are not able to prevent the structural changes. It shows that ROS are not the sole causative mechanism of hepatic RI and other mechanisms and mediators may be involved.

Keyword Hypoxia/reperfusion
Free radical scavengers
Superoxide
Isolated perfused rat liver
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
School of Veterinary Science Publications
 
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