m144, a murine cytomegalovirus (MCMV)-encoded major histocompatibility complex class I homologue, confers tumor resistance to natural killer cell-mediated rejection

Cretney, Erika, Degli-Esposti, Mariapia A., Densley, Eloise H., Farrell, Helen E., Davis-Poynter, Nick J. and Smyth, Mark J. (1999) m144, a murine cytomegalovirus (MCMV)-encoded major histocompatibility complex class I homologue, confers tumor resistance to natural killer cell-mediated rejection. Journal of Experimental Medicine, 190 3: 435-443. doi:10.1084/jem.190.3.435

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Author Cretney, Erika
Degli-Esposti, Mariapia A.
Densley, Eloise H.
Farrell, Helen E.
Davis-Poynter, Nick J.
Smyth, Mark J.
Title m144, a murine cytomegalovirus (MCMV)-encoded major histocompatibility complex class I homologue, confers tumor resistance to natural killer cell-mediated rejection
Journal name Journal of Experimental Medicine   Check publisher's open access policy
ISSN 1540-9538
0022-1007
Publication date 1999-08-02
Sub-type Article (original research)
DOI 10.1084/jem.190.3.435
Open Access Status File (Publisher version)
Volume 190
Issue 3
Start page 435
End page 443
Total pages 9
Place of publication New York
Publisher Rockefeller University Press
Language eng
Subject 060506 Virology
Abstract Until now, it has been unclear whether murine cytomegalovirus (MCMV)-encoded protein m144 directly regulates natural killer (NK) cell effector function and whether the effects of m144 are only strictly evident in the context of MCMV infection. We have generated clones of the transporter associated with antigen processing (TAP)-2-deficient RMA-S T lymphoma cell line and its parent cell line, RMA, that stably express significant and equivalent levels of m144. In vivo NK cell-mediated rejection of RMA-S-m144 lymphomas was reduced compared with rejection of parental or mock-transfected RMA-S clones, indicating the ability of m144 to regulate NK cell-mediated responses in vivo. Significantly, the accumulation of NK cells in the peritoneum was reduced in mice challenged with RMA-S-m144, as was the lytic activity of NK cells recovered from the peritoneum. Expression of m144 on RMA-S cells also conferred resistance to cytotoxicity mediated in vitro by interleukin 2-activated adherent spleen NK cells. In summary, the data demonstrate that m144 confers some protection from NK cell effector function mediated in the absence of target cell class I expression, but that in vivo the major effect of m144 is to regulate NK cell accumulation and activation at the site of immune challenge.
Keyword Immunology
Medicine, Research & Experimental
Mouse
Tumor Immunity
Natural Killer Cells
Cytotoxicity
Cytomegalovirus
Cytotoxic T-lymphocytes
Antigen Presentation
Viral-infections
Mhc Homolog
Molecules
Peptides
Binds
Gamma
Subpopulation
Requirement
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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Created: Mon, 13 Aug 2007, 11:18:09 EST