Changes in glucose fermentation pathways by an enriched bacterial culture in response to regulated dissolved H2 concentrations

Zheng, Hang, Zeng, Raymond J., Duke, Mikel C., O'Sullivan, Cathryn A. and Clarke, William P. (2015) Changes in glucose fermentation pathways by an enriched bacterial culture in response to regulated dissolved H2 concentrations. Biotechnology and Bioengineering, 112 6: 1177-1186. doi:10.1002/bit.25525


Author Zheng, Hang
Zeng, Raymond J.
Duke, Mikel C.
O'Sullivan, Cathryn A.
Clarke, William P.
Title Changes in glucose fermentation pathways by an enriched bacterial culture in response to regulated dissolved H2 concentrations
Formatted title
Changes in glucose fermentation pathways by an enriched bacterial culture in response to regulated dissolved H2 concentrations
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 1097-0290
0006-3592
Publication date 2015-06
Year available 2015
Sub-type Article (original research)
DOI 10.1002/bit.25525
Open Access Status
Volume 112
Issue 6
Start page 1177
End page 1186
Total pages 10
Place of publication Hoboken NJ, United States
Publisher John Wiley & Sons
Collection year 2016
Language eng
Formatted abstract
It is well established that metabolic pathways in the fermentation of organic waste are primarily controlled by dissolved H2 concentrations, but there is no reported study that compares observed and predicted shifts in fermentation pathways induced by manipulating the dissolved H2 concentration. A perfusion system is presented that was developed to control dissolved H2 concentrations in the continuous fermentation of glucose by a culture highly enriched towards Thermoanaerobacterium thermosaccharolyticum (86 ± 9% relative abundance) from an originally diverse consortia in the leachate of a laboratory digester fed with municipal solid waste. Media from a 2.5 L CSTR was drawn through sintered steel membrane filters to retain biomass, allowing vigorous sparging in a separate chamber without cellular disruption. Through a combination of sparging and variations in glucose feeding rate from 0.8 to 0.2 g/L/d, a range of steady state fermentations were performed with dissolved H2 concentrations as low as an equivalent equilibrated H2 partial pressure of 3 kPa. Trends in product formation rates were simulated using a H2 regulation partitioning model. The model correctly predicted the direction of products redistribution in response to H2 concentration changes and the acetate and butyrate formation rates when H2 concentrations were less than 6 kPa. However, the model over-estimated acetate, ethanol and butanol productions at the expense of butyrate production at higher H2 concentrations. The H2 yield at the lowest dissolved H2 concentration was 2.67 ± 0.08 mol H2/mol glucose, over 300% higher than the yield achieved in a CSTR operated without sparging.
Keyword Dissolved H2 concentration
H2 stripping
Metabolic pathway shift
H2 regulation partitioning model
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Civil Engineering Publications
School of Chemical Engineering Publications
Official 2016 Collection
 
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