Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability

Yoshinaga, Marcos Y., Gagen, Emma J., Wormer, Lars, Broda, Nadine K., Meador, Travis B., Wendt, Jenny, Thomm, Michael and Hinrichs, Kai-Uwe (2015) Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability. Frontiers in Microbiology, 6 JAN: 1-9. doi:10.3389/fmicb.2015.00005


Author Yoshinaga, Marcos Y.
Gagen, Emma J.
Wormer, Lars
Broda, Nadine K.
Meador, Travis B.
Wendt, Jenny
Thomm, Michael
Hinrichs, Kai-Uwe
Title Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability
Formatted title
Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability
Journal name Frontiers in Microbiology   Check publisher's open access policy
ISSN 1664-302X
Publication date 2015-01-22
Sub-type Article (original research)
DOI 10.3389/fmicb.2015.00005
Open Access Status DOI
Volume 6
Issue JAN
Start page 1
End page 9
Total pages 9
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2016
Language eng
Formatted abstract
Methanothermobacter thermautotrophicus strain ΔH is a model hydrogenotrophic methanogen, for which extensive biochemical information, including the complete genome sequence, is available. Nevertheless, at the cell membrane lipid level, little is known about the responses of this archaeon to environmental stimuli. In this study, the lipid composition of M. thermautotrophicus was characterized to verify how this archaeon modulates its cell membrane components during growth phases and in response to hydrogen depletion and nutrient limitation (potassium and phosphate). As opposed to the higher abundance of phospholipids in the stationary phase of control experiments, cell membranes under nutrient, and energy stress were dominated by glycolipids that likely provided a more effective barrier against ion leakage. We also identified particular lipid regulatory mechanisms in M. thermautotrophicus, which included the accumulation of polyprenols under hydrogen-limited conditions and an increased content of sodiated adducts of lipids in nutrient-limited cells. These findings suggest that M. thermautotrophicus intensely modulates its cell membrane lipid composition to cope with energy and nutrient availability in dynamic environments.
Keyword Archaea
Stress response
Polar lipids
Diether
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published online 22 January 2015. Article # 5

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Earth Sciences Publications
Non HERDC
 
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