High-throughput phenotyping of seminal root traits in wheat

Richard, Cecile A. I., Hickey, Lee T., Fletcher, Susan, Jennings, Raeleen, Chenu, Karine and Christopher, Jack T. (2015) High-throughput phenotyping of seminal root traits in wheat. Plant Methods, 11 13: 1-11. doi:10.1186/s13007-015-0055-9


Author Richard, Cecile A. I.
Hickey, Lee T.
Fletcher, Susan
Jennings, Raeleen
Chenu, Karine
Christopher, Jack T.
Title High-throughput phenotyping of seminal root traits in wheat
Journal name Plant Methods   Check publisher's open access policy
ISSN 1746-4811
Publication date 2015-03-01
Year available 2015
Sub-type Article (original research)
DOI 10.1186/s13007-015-0055-9
Open Access Status DOI
Volume 11
Issue 13
Start page 1
End page 11
Total pages 11
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2016
Language eng
Formatted abstract
Background

Water availability is a major limiting factor for wheat (Triticum aestivum L.) production in rain-fed agricultural systems worldwide. Root system architecture has important functional implications for the timing and extent of soil water extraction, yet selection for root architectural traits in breeding programs has been limited by a lack of suitable phenotyping methods. The aim of this research was to develop low-cost high-throughput phenotyping methods to facilitate selection for desirable root architectural traits. Here, we report two methods, one using clear pots and the other using growth pouches, to assess the angle and the number of seminal roots in wheat seedlings– two proxy traits associated with the root architecture of mature wheat plants.

Results

Both methods revealed genetic variation for seminal root angle and number in the panel of 24 wheat cultivars. The clear pot method provided higher heritability and higher genetic correlations across experiments compared to the growth pouch method. In addition, the clear pot method was more efficient – requiring less time, space, and labour compared to the growth pouch method. Therefore the clear pot method was considered the most suitable for large-scale and high-throughput screening of seedling root characteristics in crop improvement programs.

Conclusions

The clear-pot method could be easily integrated in breeding programs targeting drought tolerance to rapidly enrich breeding populations with desirable alleles. For instance, selection for narrow root angle and high number of seminal roots could lead to deeper root systems with higher branching at depth. Such root characteristics are highly desirable in wheat to cope with anticipated future climate conditions, particularly where crops rely heavily on stored soil moisture at depth, including some Australian, Indian, South American, and African cropping regions.
Keyword Wheat breeding
Root angle
Root number
Adaptation
Drought
Triticum aestivum L.
Water limited environments
Architectural traits
System architecture
Drought adaptation
Genetic variability
Canopy temperature
Eastern Australia
Grain dormancy
Yield
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2016 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 26 Apr 2015, 01:31:59 EST by System User on behalf of Qld Alliance for Agriculture and Food Innovation